Oligomerization of Baculovirus LEF-11 Is Involved in Viral DNA Replication.
Oligomerization of Baculovirus LEF-11 Is Involved in Viral DNA Replication.
复制标题
杆状病毒 LEF-11 寡聚化参与病毒 DNA 复制
DOI：
10.1371/journal.pone.0144930
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Pan MH
中科院分区：
文献类型：
--
作者：
Dong ZQ;Hu N;Zhang J;Chen TT;Cao MY;Li HQ;Lei XJ;Chen P;Lu C;Pan MH
We have previously reported that baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) late expression factor 11 (lef-11) is associated with viral DNA replication and have demonstrated that it potentially interacts with itself; however, whether LEF-11 forms oligomers and the impact of LEF-11 oligomerization on viral function have not been substantiated. In this study, we first demonstrated that LEF-11 is capable of forming oligomers. Additionally, a series of analyses using BmNPV LEF-11 truncation mutants indicated that two distinct domains control LEF-11 oligomerization (aa 42–61 and aa 72–101). LEF-11 truncation constructs were inserted into a lef-11-knockout BmNPV bacmid, which was used to demonstrate that truncated LEF-11 lacking either oligomerization domain abrogates viral DNA replication. Finally, site-directed mutagenesis was used to determine that the conserved hydrophobic residues Y58&I59 (representing Y58 and I59), I85 and L88&L89 (representing L88 and L89) are required for LEF-11 oligomerization and viral DNA replication. Collectively, these data indicate that BmNPV LEF-11 oligomerization influences viral DNA replication.
