7α-Hydroxypregnenolone, a key neuronal modulator of locomotion, stimulates upstream migration by means of the dopaminergic system in salmon.
7α-Hydroxypregnenolone, a key neuronal modulator of locomotion, stimulates upstream migration by means of the dopaminergic system in salmon.
复制标题
DOI：
10.1038/srep12546
复制
发表时间：
2015-07-29
影响因子：
4.6
通讯作者：
Tsutsui K
中科院分区：
文献类型：
--
作者：
Haraguchi S;Yamamoto Y;Suzuki Y;Hyung Chang J;Koyama T;Sato M;Mita M;Ueda H;Tsutsui K
鲑鱼在它们纳塔尔的河流中逆流而上。然而，刺激上游迁移行为的分子机制知之甚少。在这里，我们表明，7α-羟基双烯醇酮（7α-OH PREG），一个新发现的神经元运动调节剂，作为一个关键因素上游迁移的鲑鱼。我们首先在鲑鱼脑中鉴定了7α-OH PREG和细胞色素P450 7α-羟化酶（P4507α），这是一种产生7α-OH PREG的类固醇生成酶，然后发现大脑中的7α-OH PREG合成在上游迁移过程中增加。随后，我们证明了7α-OH PREG增加鲑鱼的上游迁移行为。我们进一步发现7α-OH PREG作用于视前核大细胞多巴胺神经元的上游迁移过程。因此，7α-OH PREG通过鲑鱼的多巴胺能系统刺激上游迁移行为。这些发现为鱼类向上游迁移的分子机制提供了新的见解。
Salmon migrate upstream against an opposing current in their natal river. However, the molecular mechanisms that stimulate upstream migratory behavior are poorly understood. Here, we show that 7α-hydroxypregnenolone (7α-OH PREG), a newly identified neuronal modulator of locomotion, acts as a key factor for upstream migration in salmon. We first identified 7α-OH PREG and cytochrome P450 7α-hydroxylase (P4507α), a steroidogenic enzyme producing 7α-OH PREG, in the salmon brain and then found that 7α-OH PREG synthesis in the brain increases during upstream migration. Subsequently, we demonstrated that 7α-OH PREG increases upstream migratory behavior of salmon. We further found that 7α-OH PREG acts on dopamine neurons in the magnocellular preoptic nucleus during upstream migration. Thus, 7α-OH PREG stimulates upstream migratory behavior through the dopaminergic system in salmon. These findings provide new insights into the molecular mechanisms of fish upstream migration.