APOLIPOPROTEIN-E ASSOCIATED WITH ASTROCYTIC GLIA OF THE CENTRAL NERVOUS-SYSTEM AND WITH NONMYELINATING GLIA OF THE PERIPHERAL NERVOUS-SYSTEM
APOLIPOPROTEIN-E ASSOCIATED WITH ASTROCYTIC GLIA OF THE CENTRAL NERVOUS-SYSTEM AND WITH NONMYELINATING GLIA OF THE PERIPHERAL NERVOUS-SYSTEM
复制标题
DOI：
10.1172/jci112130
复制
发表时间：
1985-01-01
影响因子：
15.9
通讯作者：
TAYLOR, JM
中科院分区：
文献类型：
--
作者：
BOYLES, JK;PITAS, RE;TAYLOR, JM
载脂蛋白E（apolipoprotein E，apo E）是哺乳动物体内脂质转运和代谢的重要决定因素。在本研究中，免疫细胞化学已被用来确定载脂蛋白E在特定细胞的中枢和外周神经系统的大鼠。光学显微镜检查显示，所有星形胶质细胞，包括专门的星形胶质细胞（小脑的Bergmann胶质细胞、第三脑室的伸长细胞、神经垂体的垂体细胞和视网膜的Muller细胞），都具有显著浓度的载脂蛋白E。在中枢神经系统的所有主要分支中，发现星形胶质细胞的核周区以及它们的细胞突起（终止于软膜表面或沿着血管的基底膜上）富含载脂蛋白E。细胞外载脂蛋白E沿着这些相同的表面。通过电子显微镜免疫细胞化学研究证实了apo E由星形胶质细胞分泌的印象，该研究表明apo E存在于高尔基体中。载脂蛋白E不存在于神经元，少突胶质细胞，小胶质细胞，室管膜细胞，脉络膜细胞。在周围神经系统中，apo E存在于感觉和运动神经元周围的胶质细胞中;背根神经节和上级颈交感神经节的卫星细胞以及肠神经节的肠胶质细胞具有反应性。载脂蛋白E也存在于非髓鞘化的雪旺细胞内，但不存在于周围神经的髓鞘化雪旺细胞内。这些结果表明，载脂蛋白E有一个重要的，以前未被怀疑的作用，在神经组织的生理。
The plasma protein apolipoprotein (apo) E is an important determinant of lipid transport and metabolism in mammals. In the present study, immunocytochemistry has been used to identify apo E in specific cells of the central and peripheral nervous systems of the rat. Light microscopic examination revealed that all astrocytes, including specialized astrocytic cells (Bergmann glia of the cerebellum, tanycytes of the third ventricle, pituicytes of the neurohypophysis, and Muller cells of the retina), possessed significant concentrations of apo E. In all of the major subdivisions of the central nervous system, the perinuclear region of astrocytic cells, as well as their cell processes that end on basement membranes at either the pial surface or along blood vessels, were found to be rich in apo E. Extracellular apo E was present along many of these same surfaces. The impression that apo E is secreted by astrocytic cells was confirmed by electron microscopic immunocytochemical studies, which demonstrated the presence of apo E in the Golgi apparatus. Apo E was not present in neurons, oligodendroglia, microglia, ependymal cells, and choroidal cells. In the peripheral nervous system, apo E was present within the glia surrounding sensory and motor neurons; satellite cells of the dorsal root ganglia and superior cervical sympathetic ganglion as well as the enteric glia of the intestinal ganglia were reactive. Apo E was also present within the non-myelinating Schwann cells but not within the myelinating Schwann cells of peripheral nerves. These results suggest that apo E has an important, previously unsuspected role in the physiology of nervous tissue.