Influenza A virus subtypes in wild birds in North-Eastern Spain (Catalonia)
Influenza A virus subtypes in wild birds in North-Eastern Spain (Catalonia)
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DOI：
10.1016/j.virusres.2009.12.005
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发表时间：
2010-04-01
期刊：
影响因子：
5
通讯作者：
Majo, Natalia
中科院分区：
文献类型：
--
作者：
Busquets, Nuria;Alba, Anna;Majo, Natalia
自2005年H5N1高致病性禽流感病毒传播以来，世界各地针对家禽和野生鸟类启动了许多监测计划。这项研究首次描述了在西地中海地区（西班牙东北部加泰罗尼亚）野生鸟类中检测到的不同亚型禽流感病毒（AIV）。在三年期间（从2006年中期到2009年中期），对来自16个不同科的1374只禽类进行了检查，通过实时逆转录酶PCR检测总共检测到了62种AIV。 AIV 在鸭科、凤凰科、鸥科和鸥科中更常见。在62个阳性样本中，28个AIV可在含胚鸡蛋中分离出来。所有分离株均通过血凝素和神经氨酸酶抑制技术进行亚型分类，并在 13 种不同的亚型组合中发现了 10 种不同的血凝素 (HA) 和 7 种神经氨酸酶 (NA)。最常见的组合是 H4N6 (22.2%) 和 H1N1 (18.5%)。将不同 AIV 亚型的 HA 和 NA 基因序列与基因组数据库中可用的 AIV 毒株进行比较和比对。我们对AIV系统发育分析的研究表明，从西班牙东北部野生鸟类测序的所有AIV基因都聚集在欧亚鸟类进化枝内，包括在欧洲首次分析的H8、N4和N5基因序列。研究结果有助于地中海地区和欧洲对 AIV 的了解。 (C) 2009 Elsevier B.V. 保留所有权利。
Since the spread of H5N1 highly pathogenic avian influenza virus in 2005, many surveillance programmes have been initiated in poultry and wild birds worldwide. This study describes for the first time the detection of different subtypes of avian influenza viruses (AIV) in wild birds in the West Mediterranean area (Catalonia, North-Eastern Spain). During a 3-year period (from mid-2006 to mid-2009), 1374 birds from 16 different families were examined, and a total of 62 AIV were detected by means of a real-time reverse transcriptase PCR assay. AIV were more frequently detected in Anatidae, Phoenicopteridae, Rallidae and Laridae families. Of the 62 positive samples, 28 AIV could be isolated in embryonated eggs. All isolates were subtyped by haemagglutinin and neuraminidase inhibition techniques and 10 different haemagglutinins (HA) and 7 neuraminidases (NA) were found in 13 different subtype combinations. The most common combinations were H4N6 (22.2%) and H1N1 (18.5%). The HA and NA gene sequences of different AIV subtypes were compared and aligned with those available AIV strains from genome databases. Our studies on AIV phylogenetic analysis revealed that all AIV genes sequenced from wild birds in North-Eastern Spain clustered within Eurasian avian clades, including the sequences of H8, N4 and N5 genes analyzed for the first time in Europe. The results contribute to the understanding of AIV in the Mediterranean area and in Europe. (C) 2009 Elsevier B.V. All rights reserved.