Adiponectin protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity
Adiponectin protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity
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DOI：
10.1016/j.bbrc.2006.02.186
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发表时间：
2006-05-05
影响因子：
3.1
通讯作者：
Cha, BS
中科院分区：
文献类型：
--
作者：
Jung, TW;Lee, JY;Cha, BS
1-甲基-4-苯基吡啶离子(MPP+)是一种线粒体复合体1的抑制剂，因其可引起严重的帕金森病样综合征，表现为细胞内活性氧水平升高和细胞凋亡性死亡，因此被广泛用作神经毒素。脂联素由脂肪组织分泌，以肝脏和肌肉为靶器官，介导全身胰岛素敏感性。脂联素还可以抑制内皮细胞中超氧化物的产生。本研究探讨脂联素对MPP+诱导的人神经母细胞瘤SH-SY5Y细胞毒性的保护作用及其机制。结果提示，脂联素对MPP+诱导的细胞凋亡具有保护作用，其机制可能与其抗氧化作用、通过诱导细胞内超氧化物歧化酶和过氧化氢酶的表达、调节细胞内的Bcl2和Bax的表达而起到抗细胞凋亡作用有关。这些数据表明，脂联素可能为帕金森病等进行性神经退行性疾病的治疗提供了一种有用的治疗策略。(C)2006 Elsevier Inc.保留所有权利。
1-Methyl-4-phenylpyridinium ion (MPP+), an inhibitor of mitochondrial complex 1, has been widely used as a neurotoxin because it elicits a severe Parkinson's disease-like syndrome characterized by elevation of intracellular reactive oxygen species level and apoptotic death. Adiponectin, secreted from adipose tissue, mediates systemic insulin sensitivity with liver and muscle as target organs. Adiponectin can also suppress superoxide generation in endothelial cells. In the present study, we investigated the protective effects of adiponectin on MPP+-induced cytotoxicity in human neuroblastoma SH-SY5Y cells, as well as the underlying mechanism. Our results suggest that the protective effects of adiponectin on MPP+-induced apoptosis may be ascribed to its anti-oxidative properties, anti-apoptotic activity via inducing expression of SOD and catalase, and regulation of Bcl-2 and Bax expression. These data indicated that adiponectin might provide a useful therapeutic strategy for the treatment of progressive neurodegenerative diseases such as Parkinson's disease. (c) 2006 Elsevier Inc. All rights reserved.