Wolbachia increase germ cell mitosis to enhance the fecundity of Laodelphax striatellus
Wolbachia increase germ cell mitosis to enhance the fecundity of Laodelphax striatellus
复制标题
沃尔巴克氏体增加生殖细胞有丝分裂，增强灰飞虱的繁殖力
DOI：
10.1016/j.ibmb.2020.103471
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发表时间：
2020-12-01
影响因子：
3.8
通讯作者：
Wu, Yu
中科院分区：
文献类型：
--
作者：
Guo, Yan;Khan, Jehangir;Wu, Yu
沃尔巴克氏体是一种细胞内细菌，感染范围广泛的无脊椎动物，并已进化出各种策略，以改变宿主的繁殖，为自己的生存和传播。在灰飞虱体内，感染沃尔巴克氏体的雌虫比未感染的雌虫产更多的卵。我们的前期研究表明，Wolbachia在L.并以半胱天冬酶依赖的方式改变凋亡的护理细胞的数量，为卵子发生提供营养。本文对Wolbachia介导的L.纹状体卵子发生仍然是未知的。在这里，我们调查了是否生殖细胞有丝分裂，这已牵连到决定卵子生产率，影响之间的相互作用的生殖力和沃尔巴克氏体在L。纹状体。我们使用抗磷酸化组蛋白3（pH 3）抗体来标记和可视化有丝分裂细胞。显微镜观察表明，沃尔巴克氏体菌株wStri增加了含有有丝分裂生殖细胞的卵巢管的数量。沃尔巴克氏体感染的女性生殖力的增加是生殖细胞有丝分裂的结果，生殖细胞有丝分裂的频率在感染的女性比未感染的女性高得多。此外，Cdc20、CDK1和CycB信使RNA干扰对Wolbachiainfinded L.纹状体显著减少卵数。活的沃尔巴克氏体灭活提高了未感染的L.通过直接影响有丝分裂调节因子来控制纹状体。总之，这些数据表明，wStri可能会增加生殖细胞有丝分裂，以提高生殖力的L。有丝分裂调节的方式。我们的研究结果建立了沃尔巴克氏体诱导的有丝分裂和沃尔巴克氏体介导的卵子产生效应之间的联系。
Wolbachia are intracellular bacteria that infect a wide range of invertebrates and have evolved various strategies to alter host reproduction for their own survival and dissemination. In small brown planthopper Laodelphax striatellus, Wolbachia-infected females lay more eggs than uninfected females. Our previous study has shown that Wolbachia are abundant in ovarian cells of L. striatellus and change the number of apoptotic nurse cells in a caspase-dependent manner to provide nutrition for oogenesis. The cellular and molecular bases of the Wolbachiamediated alterations in L. striatellus oogenesis remain largely unknown. Here, we investigated whether germ cell mitosis, which has been implicated in determination of egg production rates, influences the interaction between fecundity and Wolbachia in L. striatellus. We used an anti-phospho-histone 3 (pH3) antibody to label and visualize mitotic cells. Microscopic observations indicated that the Wolbachia strain wStri increased the number of ovarioles that contained mitotic germ cells. The increased fecundity of Wolbachia-infected females was a result of mitosis of germ cells; the frequency of germ cell mitosis was much higher in infected females than in uninfected females. In addition, mitosis inhibition by Cdc20, CDK1, and CycB messenger RNA interference in Wolbachiainfected L. striatellus markedly decreased egg numbers. Live Wolbachia recolonization enhanced the egg production of uninfected L. striatellus by directly affecting mitosis regulators. Together, these data suggest that wStri might increase germ cell mitosis to enhance the fecundity of L. striatellus in a mitosis-regulating manner. Our findings establish a link between Wolbachia-induced mitosis and Wolbachia-mediated egg production effects.