Electrospray-mass spectrometry characterization and measurement of far-UV-induced thymine photoproducts
Electrospray-mass spectrometry characterization and measurement of far-UV-induced thymine photoproducts
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DOI：
10.1016/s1011-1344(00)00009-9
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发表时间：
2000-02-01
影响因子：
5.4
通讯作者：
Cadet, J
中科院分区：
文献类型：
--
作者：
Douki, T;Court, M;Cadet, J
远紫外光诱导DNA中二聚嘧啶光产物的形成是太阳光致癌作用的主要原因。这类损伤的化学结构主要是通过对模型化合物的研究确定的。本工作旨在提供胸腺嘧啶-胸腺嘧啶光产物的质谱学数据，包括环丁烷二聚体的非对映异构体、(6-4)加合物、相关的杜瓦价异构体和孢子光产物。通过三重四极或离子陷阱检测的电喷雾电离，记录了修饰碱基、核苷、一磷酸二核苷酸和二核苷酸的裂解质谱图。结果表明，不同类型的感光产品在碎裂模式上存在差异。此外，对环丁烷二聚体的非对映等温测量也观察到了强烈的影响。建立了一种灵敏的高效液相色谱-质谱联用多反应监测检测负性模式分析单磷酸二核苷光产物的检测技术。(C)2000 Elsevier Science S.A.保留所有权利。
Far-UV-induced formation of dimeric pyrimidine photoproducts within DNA is a major cause of the carcinogenic effects of solar light. The chemical structure of this class of lesion has been mostly determined by studies on model compounds. The present work is aimed at providing mass spectrometry data on the thymine-thymine photoproducts, including the diastereoisomers of the cyclobutane dimer, the (6-4) adduct, the related Dewar valence isomer and the spore photoproduct. Fragmentation mass spectra of the modified bases, nucleosides, dinucleoside monophosphates and dinucleotides were recorded following electrospray ionization with either triple-quadrupolar or ion-trap detection. The results showed differences in fragmentation pattern between the different types of photoproducts. In addition, a drastic effect of the diastereoisometry was observed for the cyclobutane dimers. A sensitive detection technique has been developed for the analysis of dinucleoside monophosphate photoproducts by high-performance liquid chromatography associated with mass spectrometry in the negative mode with multiple reaction-monitoring detection. (C) 2000 Elsevier Science S.A. All rights reserved.