Direct Mass Spectrometry Analysis of Untreated Samples of Ultralow Amounts Using Extraction Nano-Electrospray.
Direct Mass Spectrometry Analysis of Untreated Samples of Ultralow Amounts Using Extraction Nano-Electrospray.
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DOI：
10.1039/c3ay41149d
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发表时间：
2013-12-07
期刊：
影响因子：
--
通讯作者：
Ouyang Z
中科院分区：
文献类型：
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作者：
Ren Y;Liu J;Li L;McLuckey MN;Ouyang Z
采用快速萃取和 nanoESI（电喷雾电离）相结合的方式，对体积低至 0.2 µL 的未处理样品进行直接质谱分析。纸质基材上的干燥样品中的分析物在 nanoESI 管中通过有机溶剂萃取，并通过施加高电压电离以产生喷雾。该电离源对三重四极杆仪器的不同大气压接口产生稳定的信号。整个分析过程消耗 10 µL 溶剂，分析时间超过 20 分钟。定性和定量分析的性能通过各种样品进行表征。分析河水中的莠去津、橙色匀浆中的噻菌灵和血液中的甲基苯丙胺时，检测限低至 0.1 ng/mL（相当于绝对量 0.05 pg）。
Direct mass spectrometry analysis of untreated samples of volumes as low as 0.2 µL were achieved using fast extraction and nanoESI (electrospray ionization) in a combined fashion. The analytes in dried samples on paper substrates were extracted by organic solvent in a nanoESI tube and ionized with a high voltage applied for generating a spray. The ionization source produced stable signals for different atmospheric pressure interfaces of triple quadrupole instruments. Analysis time more than 20 minutes were available with 10 µL solvent consumed for the entire analysis process. The performance in qualitative and quantitative analysis was characterized with a wide variety of samples. Limits of detection as low as 0.1 ng/mL (corresponding to an absolute amount of 0.05 pg) were obtained for analysis of atrazine in river water, thiabendazole in orange homogenate, and methamphetamine in blood.