Calcium and EGTA Alleviate Cadmium Toxicity in Germinating Chickpea Seeds
Calcium and EGTA Alleviate Cadmium Toxicity in Germinating Chickpea Seeds
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DOI：
10.1007/s00344-016-9605-2
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发表时间：
2016-05
影响因子：
4.8
通讯作者：
Lamia Sakouhi;Sondès Rahoui;M. Ben Massoud;S. Munemasa;Ezzeddine El Ferjani;Y. Murata;A. Chaoui
中科院分区：
文献类型：
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作者：
Lamia Sakouhi;Sondès Rahoui;M. Ben Massoud;S. Munemasa;Ezzeddine El Ferjani;Y. Murata;A. Chaoui
外源钙和EGTA对鹰嘴豆生长发育的影响研究了暴露于镉胁迫6天的发芽种子。钙和EGTA后期处理（3天）缓解生长抑制，降低镉积累以及脂质过氧化和蛋白质羰基化在根和地上部细胞。外源性效应物的应用缓解镉诱导的细胞死亡，这是与一个恒定的ATP水平，这被认为是一个类似于细胞凋亡的过程。通过研究吡啶核苷酸对NAD+/NADH和NADP+/NADPH的氧化还原状态以及它们相关的氧化[NAD（P）H-氧化酶]和脱氢酶（葡萄糖-6-磷酸脱氢酶、6-磷酸葡萄糖酸脱氢酶和苹果酸脱氢酶）酶活性来检查氧化还原平衡。本研究表明，钙和EGTA对生长的镉暴露鹰嘴豆幼苗，发生通过保护敏感的细胞部位，从镉诱导的氧化，即膜脂和蛋白质，而不是提高细胞的还原能力的再循环能力的改善。
Impact of exogenous calcium and ethylene glycol tetraacetic acid (EGTA) supplement on chickpea (Cicer arietinumL.) germinating seeds exposed to cadmium stress for 6 days was studied. Ca and EGTA late treatment (3 days) alleviated growth inhibition and decreased Cd accumulation as well as lipid peroxidation and protein carbonylation in both root and shoot cells. Exogenous effector application relieved Cd-induced cell death which was associated with a constant level of ATP, which was considered as an apoptotic-like process. Redox balance was examined through the study of the redox state of pyridine nucleotide couples NAD+/NADH and NADP+/NADPH as well as their related oxidative [NAD(P)H-oxidase] and dehydrogenase (glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and malate dehydrogenase) enzyme activities. The present research illustrated an ameliorative effect of Ca and EGTA on growth of Cd-exposed chickpea seedlings that occurs through the protection of sensitive cell sites from Cd-induced oxidation, namely membrane lipids and proteins, rather than the improvement of recycling capabilities of the cellular reducing power.