Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
复制标题
巯基、二硫化物试剂和钾离子对 Lowry 法蛋白质测定的干扰。
DOI：
10.1016/0003-2697(70)90349-0
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发表时间：
1970
影响因子：
2.9
通讯作者：
Rosario Lagunas
中科院分区：
文献类型：
--
作者：
Carmen G. Vallejo;Rosario Lagunas
巯基、二硫试剂和钾离子干扰洛瑞法测定蛋白质。给出了这些物质在使用时不会受到严重干扰的浓度。巯基和二硫试剂产生的颜色与蛋白质产生的颜色具有相同的吸收光谱。在适度的浓度下，这种干扰可以通过运行适当的空白来克服。钾离子产生白色沉淀物，可在显色后将反应混合物离心除去而不改变颜色强度。
Sulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method. The concentrations of these substances which can be used without serious interference are given. Sulfhydryl and disulfide reagents yield a color with the same absorption spectrum as that caused by proteins. At moderate concentrations this interference can be overcome by running appropriate blanks. Potassium ions produce a white precipitate that can be removed without change of color intensity by centrifuging the reaction mixtures after color development.