Changes in insulin, glucose and ketone bodies, but not leptin or body fat content precede restoration of luteinising hormone secretion in ewes.
Changes in insulin, glucose and ketone bodies, but not leptin or body fat content precede restoration of luteinising hormone secretion in ewes.
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在母羊黄体生成素分泌恢复之前，胰岛素、葡萄糖和酮体会发生变化，但瘦素或体脂肪含量不会发生变化。
DOI：
10.1111/j.1365-2826.2007.01551.x
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发表时间：
2007
影响因子：
3.2
通讯作者：
Clarke,IJ
中科院分区：
文献类型：
--
作者：
Szymanski,LA;Schneider,JE;Friedman,MI;Ji,H;Kurose,Y;Blache,D;Rao,A;Dunshea,FR;Clarke,IJ
生殖系统，包括搏动性黄体生成素(黄体生成素)的分泌，受到能量供应不足的抑制，并因能量过剩而恢复。测定了去卵巢、限食母羊饲养前后血浆中促黄体生成素、胰岛素、瘦素、生长激素、葡萄糖、酮体和非酯化脂肪酸的浓度，以确定恢复搏动性促黄体生成素分泌的影响因素。在07.00 h，每10 分钟采血一次，连续5 h，采自卵巢切除、性腺功能减退、长期食物受限和自由采血的母羊(FED)。在12：00 h，4只限食绵羊获得食物(重新饲喂)，而3只母羊继续被限食(限制)。采样持续5 h，并在第2、4和9天的早晨再次恢复。所有重新饲喂的母羊在重新饲喂后的1 h内均可观察到一次黄体生成素的脉冲，并且在重新饲喂期间，重新饲喂的母羊的脉冲间期显著短于限制饲喂的母羊，而长于饲喂的母羊。复饲LHIPI直到第4天和第9天之间才恢复到FED母羊的水平。第一次脉冲发生在几分钟内，而IPI的恢复发生在4-8 天。在重新喂养的母羊出现最初的促黄体生成素脉冲之前，血浆酮小体首先下降，然后上升到显著高于限制饲养的母羊的水平。在第4天和第9天之间的某个时间，在恢复再喂养的LHIPI之前，循环中的胰岛素、Ghrelin、葡萄糖和总酮体浓度、每天的食物摄入量和瘦体质量发生了显著的变化，但肥胖症和循环中的瘦素浓度没有显著的变化，这与这样的假设相一致，即可氧化代谢燃料和可能的胰岛素的可获得性的变化而不是瘦素浓度的变化重新启动了LH脉冲。
The reproductive system, including pulsatile luteinising hormone (LH) secretion, is inhibited by deficits in energy availability and restored by energy surfeits. Plasma LH, insulin, leptin, ghrelin, glucose, ketone body, and nonesterified fatty acid concentrations were measured in ovariectomised, food‐restricted ewes before and after return toad libitumfeeding to determine the factors that change in time to account for the restoration of pulsatile LH secretion. At 07.00 h, blood was sampled every 10 min for 5 h from ovariectomised, hypogonadotrophic, chronically food‐restricted andad libitum‐fed ewes (Fed). At 12.00 h, four of the food‐restricted sheep were givenad libitumaccess to food (Re‐Fed), while three ewes continued to be food restricted (Restricted). Sampling continued for 5 h and resumed again on the mornings of days 2, 4, and 9. A pulse of LH was seen within 1 h of re‐feeding in all Re‐Fed ewes, and interpulse interval (IPI) was significantly shorter in Re‐Fed compared to Restricted ewes and longer than in Fed ewes during the period after re‐feeding. Re‐Fed LH IPI was not restored to that of Fed ewes until sometime between days 4 and 9. The first pulse occurred within minutes, whereas restoration of IPI occurred after 4–8 days. Prior to the initial LH pulses seen in Re‐Fed ewes, plasma ketone bodies first fell and then rose to levels significantly above those in Restricted ewes. Significant changes in circulating insulin, ghrelin, glucose, and total ketone body concentrations, daily food intake and lean body mass preceded restoration of Re‐Fed LH IPI some time between days 4 and 9, but there were no significant changes in adiposity or circulating leptin concentrations, consistent with the hypothesis that LH pulses are reinitiated by changes in the availability of oxidisable metabolic fuels and possibly insulin, but not leptin concentrations.