Dynamics of mitochondria during the cell cycle
Dynamics of mitochondria during the cell cycle
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DOI：
10.1248/bpb.29.1962
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发表时间：
2006-09-01
影响因子：
2
通讯作者：
Higuti, Tomihiko
中科院分区：
文献类型：
--
作者：
Arakaki, Naokatu;Nishihama, Takeshi;Higuti, Tomihiko
线粒体是真核细胞中高度动态的细胞器。虽然线粒体在新陈代谢、ATP合成和细胞凋亡中的作用得到了更广泛的认识，但线粒体的形态和丰度的变化对细胞功能也是重要的。在这里，我们研究了同步的HeLa细胞中线粒体的动力学，其中观察细胞的主要细胞周期阶段是通过磷酸化组蛋白H1和H3染色来分辨的，并表明线粒体在细胞周期进程中以丝状网络结构存在，改变了它们的形态、分布和丰度。目前的结果表明，线粒体分裂的正常进行需要发生在前期的线粒体凝聚。
Mitochondria are highly dynamic organelles in eukaryotic cells. Although the role of mitochondria in metabolism, ATP production and apoptosis is more widely recognized, alterations in mitochondrial morphology and abundance are also important for cellular functions. Here we investigated mitochondrial dynamics in synchronized HeLa cells in which the major stages of the cell cycle of the observed cells were resolved by staining phosphorylate histones H1 and H3, and showed that mitochondria exist as filamentous network structures throughout the cell cycle progression, changing their morphology, distribution, and abundance. The current results suggest that mitochondrial condensation occurred at prophase is required for the proper progression of mitochondrial division.