Evidence for two promoters upstream of the pts operon: regulation by the cAMP receptor protein regulatory complex.
Evidence for two promoters upstream of the pts operon: regulation by the cAMP receptor protein regulatory complex.
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pts 操纵子上游两个启动子的证据：cAMP 受体蛋白调节复合物的调节。
DOI：
10.1073/pnas.89.15.7056
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发表时间：
1992
影响因子：
11.1
通讯作者：
Garges,S
中科院分区：
文献类型：
--
作者：
Fox,DK;Presper,KA;Adhya,S;Roseman,S;Garges,S
先前在pts操纵子的5'侧区确定了多种调控机制的几个潜在靶点。我们研究了cAMP受体蛋白（CRP）在体外的相互作用。cAMP调控复合体具有两个DNA结合位点，通过凝胶迁移位移测定，并报告了每个结合位点在体内的功能作用分析。启动子报告基因融合研究鉴定出两种CRP。camp依赖性启动子（先前确定的P1和另一个启动子P0）位于ptsH上游。ptsI内的crr启动子P2可能受到CRP.cAMP的负调控。
Several potential target sites for multiple regulatory mechanisms were previously identified in the 5' flanking region of the pts operon. We have investigated the in vitro interactions of the cAMP receptor protein (CRP).cAMP regulatory complex with two DNA binding sites, by gel mobility-shift assays, and report the analysis of the functional role of each of the binding sites in vivo. Promoter-reporter gene fusion studies identified two CRP.cAMP-dependent promoters (the previously identified P1 and another promoter, P0) upstream of ptsH. The crr promoters (P2) within ptsI may be negatively regulated by CRP.cAMP.