Chemical transformation of Chinese hamster cells: II. Appearance of marker chromosomes in transformed cells.
Chemical transformation of Chinese hamster cells: II. Appearance of marker chromosomes in transformed cells.
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DOI：
10.1038/bjc.1976.136
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发表时间：
1976-08
影响因子：
8.8
通讯作者：
Venitt, S
中科院分区：
文献类型：
--
作者：
Kirkland, D J;Venitt, S
对12个培养的中国仓鼠肾细胞和前列腺细胞样品（4个正常和8个转化细胞）的染色体进行了数量变化和异常标记物的检查，这些细胞的组织培养特性已被描述（柯克兰，1976）。6个转化的肾亚克隆含有正常亲本细胞中不存在的一致的端部着丝粒标记，Giemsa显带表明这是所有情况下的4号染色体的着丝粒和长臂（q）。两个转化的前列腺亚克隆也含有一致的端着丝粒标记，在类似衍生的正常亚克隆或正常亲本细胞中不存在，Giemsa显带表明这是4号染色体的不同片段（着丝粒和大部分p臂）。据信，使用旨在稳定培养的中国仓鼠细胞核型的混合血清培养基至少部分负责检测这些转化相关染色体变化。
The chromosomes of 12 samples of cultured Chinese hamster kidney and prostate cells (4 normal and 8 transformed), whose tissue culture properties have already been described (Kirkland, 1976) have been examined for numerical change and for the appearance of abnormal markers. Six transformed kidney subclones contained a consistent telocentric marker not present in the normal parental cell, and Giemsa banding demonstrated this to be the centromere and the long (q) arm of the number 4 chromosome in all cases. Two transformed prostate subclones also contained a consistent telocentric marker, not present in similarly derived normal subclones or in the normal parental cell, and Giemsa banding demonstrated this to be a different fragment (the centromere and most of the p arm) of the number 4 chromosome. It is believed that the use of a mixed-serum culture medium, designed to stabilize the karyotype of cultured Chinese hamster cells, is at least partly responsible for the detection of these transformation-associated chromosome changes.