In vivo quantum dot Labeling of mammalian stem and progenitor cells
In vivo quantum dot Labeling of mammalian stem and progenitor cells
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DOI：
10.1002/dvdy.21235
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发表时间：
2007-12-01
影响因子：
2.5
通讯作者：
Haydar, Tarik F.
中科院分区：
文献类型：
--
作者：
Slotkin, Jonathan R.;Chakrabarti, Lina;Haydar, Tarik F.
荧光半导体纳米晶量子点是一类具有多种功能的无机荧光材料，在临床和生物医学研究中具有广阔的应用前景。由于目前还没有在体内直接标记神经系统中哺乳动物细胞的方法，我们开发了新的宫内电穿孔和超声引导的体内递送技术，以高效和直接地标记发育中的哺乳动物中枢神经系统的神经干细胞和祖细胞(NSPC)。我们对单细胞和两细胞量子点标记的小鼠胚胎的初步安全性和概念验证研究表明，量子点与哺乳动物早期胚胎发育是相容的。我们的体内实验进一步表明，在子宫内标记的NSPC继续以明显正常的方式发育。这些研究表明，量子点可以有效地标记哺乳动物体内的NSPC，并将有助于研究体内命运图谱、细胞迁移和NSPC在哺乳动物发育过程中的分化。发展动力学236：3393-3401,2007。(C)2007年Wiley-Liss，Inc.
Fluorescent semiconductor nanocrystal quantum dots (QDs) are a class of multifunctional inorganic fluorophores that hold great promise for clinical applications and biomedical research. Because no methods currently exist for directed QD-labeling of mammalian cells in the nervous system in vivo, we developed novel in utero electroporation and ultrasound-guided in vivo delivery techniques to efficiently and directly label neural stem and progenitor cells (NSPCs) of the developing mammalian central nervous system with QDs. Our initial safety and proof of concept studies of one and two-cell QD-labeled mouse embryos reveal that QDs are compatible with early mammalian embryonic development. Our in vivo experiments further show that in utero labeled NSPCs continue to develop in an apparent normal manner. These studies reveal that QDs can be effectively used to label mammalian NSPCs in vivo and will be useful for studies of in vivo fate mapping, cellular migration, and NSPC differentiation during mammalian development. Developmental Dynamics 236:3393-3401, 2007. (c) 2007 Wiley-Liss, Inc.