Relationship between myocardial ultrasonic integrated backscatter and mitochondria of the myocardium in dogs
Relationship between myocardial ultrasonic integrated backscatter and mitochondria of the myocardium in dogs
复制标题
犬心肌超声积分背向散射与心肌线粒体的关系
DOI：
10.1016/j.clinimag.2006.08.021
复制
发表时间：
2006-11-01
期刊：
影响因子：
2.1
通讯作者：
Pu, Shi Yu
中科院分区：
文献类型：
--
作者：
Gong, Lin;Wang, Zhi Gang;Pu, Shi Yu
目的：本研究旨在探讨停滞心肌中积分反向散射（IBS）与线粒体之间的关系。方法：12只开胸犬随机分为两组：一组为收缩期心脏骤停，另一组为舒张期心脏骤停。胸骨旁乳头肌短轴视图的 IBS 图像存储在不同时间范围（心脏骤停后 0、30 和 60 分钟）。超声 IBS 值是使用声密度测定技术获得的。超声检查后，采集相应时间的组织样本并在透射电镜下观察。使用计算机成像分析系统对显微图像进行分析，以获得线粒体的体视学参数。分析IBS与体视学参数之间的相关性。结果：心脏骤停后，随着时间的推移，在一些肌纤维中可以观察到具有电子透明基质的肿胀线粒体。 IBS 的变化与线粒体体视参数的变化密切相关，例如体积密度 (Vv)、表面密度 (Sv)、平均体积（(nu) 上方条形）、平均表面积（(s) 上方条形）和比表面积（Rsv，表面积与体积之比）。结论：线粒体可能是 IBS 心肌中的重要散射体。 (C) 2006 Elsevier Inc. 保留所有权利。
Objective: This study aimed to explore the relationship between integrated backscatter (IBS) and mitochondria in arrested myocardium. Methods: Twelve open-chest dogs were randomly divided into two groups: one group with cardiac arrest in systole and the other with cardiac arrest in diastole. IBS images at parasternal papillary muscle short-axis view were stored at different time frames (0, 30, and 60 min after cardiac arrest). The values of ultrasonic IBS were obtained using the acoustic densitometry technique. After ultrasound examination, tissue samples of corresponding times were harvested and observed under the transmission electron microscope. The microscopic images were analyzed using a computer imaging analysis system to obtain the stereological parameters of mitochondria. The correlation between IBS and the stereological parameters was analyzed. Results: After cardiac arrest, swollen mitochondria with electron-lucent matrix could be observed in some myofibers following the progression of time. The alteration of IBS correlated well with that of mitochondrial stereological parameters, such as volume density (Vv), surface density (Sv), average volume ((nu) over bar), average surface area ((s) over bar), and specific surface (Rsv, ratio between surface and volume). Conclusion: Mitochondria might be an important scatterer in the myocardium for IBS. (C) 2006 Elsevier Inc. All rights reserved.