QUANTITATION OF A WHOLE-BLOOD ASSAY FOR HUMAN NATURAL-KILLER CELL-ACTIVITY
QUANTITATION OF A WHOLE-BLOOD ASSAY FOR HUMAN NATURAL-KILLER CELL-ACTIVITY
复制标题
DOI：
10.1016/0022-1759(81)90159-9
复制
发表时间：
1981-01-01
影响因子：
2.2
通讯作者：
BAINES, MG
中科院分区：
文献类型：
--
作者：
OTTENHOF, PC;MORALES, A;BAINES, MG
本文介绍了一种用肝素化全血进行~(51)Cr释放试验测定人自然杀伤细胞活性的方法。分离重组实验表明，去除Fc受体的淋巴细胞可消除细胞毒活性，但不能消除单核细胞和粒细胞。自体或混合血浆不具有固有的细胞溶解活性。类似于酶动力学反应，结果被表示为动力学裂解单位(Klu)，其定义为每毫升全血每单位时间可裂解的最大靶细胞数。BCC法快速、简便，适用于天然细胞毒性的筛选和监测。由于这种方法保留了自然杀伤细胞的实际浓度，它可能更真实地反映了体内的事件。
A method is described for the measurement of human natural killer cell activity using heparinized whole blood in a 51Cr release assay. Fractionation-reconstitution experiments showed that the cytotoxic activity was abolished by removal of the Fc receptor bearing lymphocytes, but not by the elimination of monocytes and granulocytes. Autologous or pooled plasma did not possess inherent cytolytic activity. By analogy to an enzyme kinetic reaction, the results were expressed as kinetic lytic units (KLU) which were defined as the maximum target cell number that could be lysed/unit time per ml whole blood. The buffy coat cytotoxicity (BCC) assay is quick, easy to perform and suitable for screening and monitoring of natural cytotoxicity. Since this methodology preserves the actual natural killer cell concentration, it may represent a truer reflection of in vivo events.