Peptide-Functionalized Quantum Dots for Rapid Label-Free Sensing of 2,4,6-Trinitrotoluene.
Peptide-Functionalized Quantum Dots for Rapid Label-Free Sensing of 2,4,6-Trinitrotoluene.
复制标题
DOI：
10.1021/acs.bioconjchem.0c00117
复制
发表时间：
2020-04
影响因子：
4.7
通讯作者：
Takumi Komikawa;Masayoshi Tanaka;Abiral Tamang;S. Evans;K. Critchley;M. Okochi
中科院分区：
文献类型：
--
作者：
Takumi Komikawa;Masayoshi Tanaka;Abiral Tamang;S. Evans;K. Critchley;M. Okochi
2,4,6-三硝基甲苯（TNT）等爆炸性化合物在全球公共安全和环境保护方面引起了极大关注。据估计，全世界有数百个 TNT 污染地点，这将影响人类、野生动物和生态系统的健康。显然，检测土壤、供水和废水中 TNT 的能力对于环境研究很重要，而且对于港口和边境等地区的安全也很重要。然而，传统的光谱检测对于现场传感来说并不实用，因为它需要复杂的设备和训练有素的人员。我们通过使用与荧光 CdTe/CdS 量子点 (QD) 缀合的 TNT 结合肽，报告了一种快速、简单的 TNT 化学传感器。量子点在水相中合成，肽通过硫醇基团直接附着在量子点表面。 QD 的荧光发射因 TNT 的添加而被猝灭。这种反应甚至可以通过肉眼观察到。荧光光谱测量的检测限估计约为 375 nM。除了快速响应（几秒钟内）之外，还证明了选择性检测。我们相信这种无标记化学传感器有助于现场爆炸物传感的进步。
Explosive compounds, such as 2,4,6-trinitrotoluene (TNT), pose a great concern in terms of both global public security and environmental protection. There are estimated to be hundreds of TNT contaminated sites all over the world, which will affect the health of humans, wildlife, and the ecosystem. Clearly, the ability to detect TNT in soils, water supplies, and wastewater is important for environmental studies but also important for security, such as in ports and boarders. However, conventional spectroscopic detection is not practical for on-site sensing because it requires sophisticated equipment and trained personnel. We report a rapid and simple chemical sensor for TNT by using TNT binding peptides which are conjugated to fluorescent CdTe/CdS quantum dots (QDs). QDs were synthesized in the aqueous phase, and the peptide was attached directly to the surface of the QDs by using thiol groups. The fluorescent emission from the QDs was quenched in response to the addition of TNT. The response could even be observed by the naked eye. The limit of detection from fluorescence spectroscopic measurement was estimated to be approximately 375 nM. In addition to the rapid response (within a few seconds), selective detection was demonstrated. We believe this label-free chemical sensor contributes to progress for the on-site explosive sensing.