DNA Methylation as Surrogate Marker For Gastric Cancer.
DNA Methylation as Surrogate Marker For Gastric Cancer.
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DOI：
10.15430/jcp.2015.20.3.172
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发表时间：
2015-09
影响因子：
2.5
通讯作者：
Rhyu MG
中科院分区：
文献类型：
--
作者：
Oh JH;Jung SH;Hong SJ;Rhyu MG
胃癌在东亚仍然顽固地居高不下。尽管如此，几乎没有可以预测胃癌的生物标志物。幽门螺杆菌感染是一种已知的胃癌致癌物，由于其相关的胃萎缩导致的恶劣的粘膜环境，通常在癌症诊断之前没有被检测到。在此，我们提出了一种基于内窥镜活检的癌症预测DNA甲基化标记。我们通过与幽门螺杆菌相关的放射性同位素标记甲基化特异性聚合酶链式反应半定量检测了CpG岛边缘附近的甲基化可变点，Hp增加了胃粘膜CpG岛上与年龄相关的过度甲基化。这些与年龄相关的过渡-CpG位点甲基化模式被认为是胃癌有用的替代标记。这将有助于为高危人群设定最佳的筛查间隔，以及评估早期胃癌患者的预后和对早期胃癌黏膜下清扫术后复发的预测性。新的胃癌筛查间隔指南应该考虑基于年龄、萎缩严重程度、幽门螺杆菌感染状况和DNA甲基化模式的个体风险。
Stomach cancer remains, stubbornly, highly prevalent in East Asia. Still, stomach cancer has few biomarkers by which it can be predicted. Helicobacter pylori infection, a known carcinogen of stomach cancer, usually goes undetected prior to cancer diagnosis, due to the poor mucosal environments that its related gastric atrophy causes. We propose, herein, an endoscopic-biopsy-based cancer-predicting DNA methylation marker. We semi-quantitatively examined the methylation-variable sites near the CpG-island margins by radioisotope-labeling methylation-specific polymerase chain reaction in association with H. pylori, which increases age-related over-methylation in CpG islands of gastric mucosa. These age-related methylation patterns of the transitional-CpG sites are proposed as useful surrogate markers for stomach cancer. It would be helpful for setting the optimal screening interval for high-risk subjects as well as for estimating the prognosis and the predictability for recurrence of early gastric cancer in patients having undergone endoscopic submucosal dissection. New screening-interval guidelines for gastric cancer should be suggested considering individual risk based on age, severity of atrophy, H. pylori status, and DNA methylation pattern.