Effects of virgin olive oil phenolics on scavenging of reactive nitrogen species and upon nitrergic neurotransmission
Effects of virgin olive oil phenolics on scavenging of reactive nitrogen species and upon nitrergic neurotransmission
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DOI：
10.1016/s0024-3205(01)01218-8
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发表时间：
2001-07-27
期刊：
影响因子：
6.1
通讯作者：
Hoult, JRS
中科院分区：
文献类型：
--
作者：
de la Puerta, R;Domínguez, MEM;Hoult, JRS
初榨橄榄油极性组分中的主要酚类物质（咖啡酸、橄榄苦苷、酪醇和羟基酪醇）具有公认的抗氧化活性，但它们对活性氮和氮能神经传递的影响尚未得到充分研究。这三种儿茶酚化合物作为硝普钠分解自发产生的一氧化氮的清除剂是有活性的（在75 μ M时达到约50%的抑制），并且具有类似的能力来清除化学上产生的过氧亚硝酸盐，如通过α（1）-抗蛋白酶失活测定所测定的（当以1 mM加入时减少67.2%-92.4%）。酪醇在这些试验中活性较低，但不具有儿茶酚官能团。尽管它们能够与化学制备的一氧化氮相互作用，但5 μ M的橄榄苦苷和羟基酪醇都不能改变神经刺激的大鼠肛尾肌制剂的NO介导的松弛，但这可能是因为氮能递质受到保护，不受外部应用的清除剂的影响。总之，在初榨橄榄油中发现的酚类物质具有抑制与人类病理学有关的活性氧和氮物质的能力，但它们的影响可能仅限于细胞外环境中存在的那些物质。(C)2001 Elsevier Science Inc. All rights reserved.
The major phenolics from the polar fraction of virgin olive oil (caffeic acid, oleuropein, tyrosol and hydroxytyrosol) have well-established antioxidant activities but their effects on reactive nitrogen species and nitrergic neurotransmission have not been fully investigated. The three catechol compounds were active as scavengers of nitric oxide generated spontaneously from the decomposition of sodium nitroprusside (approximate to 50% inhibition achieved at 75 muM), and had similar ability to scavenge chemically generated peroxynitrite, as determined by an alpha (1)-antiproteinase inactivation assay (67.2 %-92.4 % reduction when added at 1mM). Tyrosol was less active in these tests, but does not possess the catechol functionality. Despite their ability to interact with chemically prepared nitric oxide, neither oleuropein nor hydroxytyrosol at 5 muM altered NO. -mediated relaxations of the nerve-stimulated rat anococcygeus preparation, but this may be because the nitrergic transmitter is protected from the effects of externally applied scavengers. In conclusion, the phenolics found in virgin olive oil possess ability to scavenge reactive oxygen and nitrogen species that are implicated in human pathologies, but their impact may be restricted to those species present in the extracellular environment. (C) 2001 Elsevier Science Inc. All rights reserved.