On the Mechanism of Trolox as Antiblinking and Antibleaching Reagent
On the Mechanism of Trolox as Antiblinking and Antibleaching Reagent
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DOI：
10.1021/ja809117z
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发表时间：
2009-04-15
影响因子：
15
通讯作者：
Tinnefeld, Philip
中科院分区：
文献类型：
--
作者：
Cordes, Thorben;Vogelsang, Jan;Tinnefeld, Philip
光漂白和眨眼预防方面的最新进展有助于单分子和超分辨率荧光显微镜的发展。然而，抗褪色剂如Trolox的共同作用机制仍然缺失。在这篇文章中，我们提出的证据表明，Trolox的行为按照一种机制，涉及通过电子转移和随后的回收所产生的自由基离子的互补氧化还原反应的三重猝灭。这种基于还原和氧化系统（ROXS）的统一机制所需的氧化剂通过与分子氧的（光）反应形成。我们目前的证据表明，这种氧化形式是一种醌衍生物的Trolox具有强氧化性能。这些发现揭示了许多矛盾的结果，关于防褪色剂的行动，并可能导致一个共同的机制理解的光漂白及其预防。最后，提供了正确使用Trolox作为防褪色剂的配方。
Recent advances in photobleaching and blinking prevention have aided the advancement of single-molecule and super-resolution fluorescence microscopy. However, a common mechanism of the action of antifading agents such as Trolox is still missing. In this communication we present evidence that Trolox acts in accordance with a mechanism that involves triplet quenching through electron transfer and subsequent recovery of the resulting radical ion by the complementary redox reaction. The required oxidant for this unifying mechanism based on a reducing and oxidizing system (ROXS) is formed via (photo-) reaction with molecular oxygen. We present evidence that this oxidized form is a quinone derivative of Trolox with strong oxidizing properties. These findings shed light on many contradicting results regarding the action of antifading agents and might lead to a common mechanistic understanding of photobleaching and its prevention. Finally, a recipe on the proper use of Trolox as an antifading agent is provided.