Ultrasonic backscatter coefficient quantitative estimates from high-concentration Chinese hamster ovary cell pellet biophantoms
Ultrasonic backscatter coefficient quantitative estimates from high-concentration Chinese hamster ovary cell pellet biophantoms
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DOI：
10.1121/1.3655879
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发表时间：
2011-12-01
影响因子：
2.4
通讯作者：
O'Brien, William D., Jr.
中科院分区：
文献类型：
--
作者：
Han, Aiguo;Abuhabsah, Rami;O'Brien, William D., Jr.
以前的工作估计了低浓度（体积密度< 3%）中国仓鼠卵巢（CHO，6.7 μ m细胞半径）细胞团的超声背向散射系数（BSC）。这项研究将工作扩展到更高的细胞浓度（体积）（密度：9.6%至63%）。在低浓度下，BSC幅度与细胞浓度成正比，BSC频率依赖性与细胞浓度无关。在高细胞浓度下，BSC幅度与细胞浓度不成比例，BSC频率依赖性取决于细胞浓度。当体积密度达到10%至30%时，发生这种转变。在高细胞浓度条件下，BSC幅度的增加慢于在低频率（ka < 1）下与数密度成比例的增加，如其他人所观察到的。然而，新的是BSC幅度可以比高频（ka > 1）下的数密度成比例地更慢或更快地增加。同心球模型最小二乘估计值显示，随着数量密度的增加，估计的细胞半径减小，这表明随着浓度的增加，同心球模型变得不太适用，因为估计的细胞半径变得小于测量的细胞半径。从模型变得不太适用时开始，临界体积密度估计为10%至30%的细胞体积密度。(C)2011年美国声学学会。[DOI 10.1121/1.3655879]
Previous work estimated the ultrasonic backscatter coefficient (BSC) from low-concentration (volume density < 3%) Chinese Hamster Ovary (CHO, 6.7 -mu m cell radius) cell pellets. This study extends the work to higher cell concentrations (volume (densities: 9.6% to 63%). At low concentration, BSC magnitude is proportional to the cell concentration and BSC frequency dependency is independent of cell concentration. At high cell concentration, BSC magnitude is not proportional to cell concentration and BSC frequency dependency is dependent on cell concentration. This transition occurs when the volume density reaches between 10% and 30%. Under high cell concentration conditions, the BSC magnitude increases slower than proportionally with the number density at low frequencies (ka < 1), as observed by others. However, what is new is that the BSC magnitude can increase either slower or faster than proportionally with number density at high frequencies (ka > 1). The concentric sphere model least squares estimates show a decrease in estimated cell radius with number density, suggesting that the concentric spheres model is becoming less applicable as concentration increases because the estimated cell radius becomes smaller than that measured. The critical volume density, starting from when the model becomes less applicable, is estimated to be between 10% and 30% cell volume density. (C) 2011 Acoustical Society of America. [DOI: 10.1121/1.3655879]