Drosophila Fezf coordinates laminar-specific connectivity through cell-intrinsic and cell-extrinsic mechanisms.
Drosophila Fezf coordinates laminar-specific connectivity through cell-intrinsic and cell-extrinsic mechanisms.
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DOI：
10.7554/elife.33962
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发表时间：
2018-03-07
期刊：
影响因子：
7.7
通讯作者：
Pecot MY
中科院分区：
文献类型：
--
作者：
Peng J;Santiago IJ;Ahn C;Gur B;Tsui CK;Su Z;Xu C;Karakhanyan A;Silies M;Pecot MY
神经连接的层状排列是神经回路组织的基本特征。因此，识别在正确的层内协调神经连接的机制对于理解神经回路是如何组装的至关重要。在果蝇视觉系统的髓质中，神经元在十个平行层内形成连接。M3层接收来自两种神经元类型的输入，这两种神经元类型在发育过程中依次支配M3。在这里，我们表明，M3特定的神经支配的两个神经元是协调果蝇Fezf（dFezf），一个保守的转录因子，选择性地表达的早期目标输入神经元。在该细胞中，dFezf指示层特异性并激活调节另一个输入神经元的层特异性的分泌分子（Netrin）的表达。我们提出，采用转录模块，细胞固有的目标神经元到特定的层，细胞外招募其他神经元是一个一般的机制，建立分层网络的神经连接。
Laminar arrangement of neural connections is a fundamental feature of neural circuit organization. Identifying mechanisms that coordinate neural connections within correct layers is thus vital for understanding how neural circuits are assembled. In the medulla of the Drosophila visual system neurons form connections within ten parallel layers. The M3 layer receives input from two neuron types that sequentially innervate M3 during development. Here we show that M3-specific innervation by both neurons is coordinated by Drosophila Fezf (dFezf), a conserved transcription factor that is selectively expressed by the earlier targeting input neuron. In this cell, dFezf instructs layer specificity and activates the expression of a secreted molecule (Netrin) that regulates the layer specificity of the other input neuron. We propose that employment of transcriptional modules that cell-intrinsically target neurons to specific layers, and cell-extrinsically recruit other neurons is a general mechanism for building layered networks of neural connections.