An improved protocol for separating meiofauna from sediments using colloidal silica sols
An improved protocol for separating meiofauna from sediments using colloidal silica sols
复制标题
DOI：
10.3354/meps214161
复制
发表时间：
2001-01-01
影响因子：
2.5
通讯作者：
Burgess, B
中科院分区：
文献类型：
--
作者：
Burgess, B
为了最大限度地减少沉积物粒度测定对底栖动物提取效率的影响，开发了一种新的等重分离技术。该方法使用胶体二氧化硅Ludox((R)) (DuPont) HS 40作为高密度溶液，从沉积物中提取微量生物。以前使用二氧化硅溶胶的方法在沉积物和标本之间的物理相互作用方面存在问题，导致沉积物类型之间的提取效率存在很大差异。本文提出的程序通过增加5分钟的沉淀物流化时间来减少这个问题。完成该方法所用的时间与最后的离心步骤最小化，这加快了细沉积物的沉降速率，导致分离所需的总时间约为20分钟。48个样本被处理，沉积物从沙子到粉质粘土不等。平均提取效率为96.8 +/- 3.9%。在测试的沉积物范围内，萃取效率与沉积物粒度无关。
A new isopycnic separation technique was developed to minimize the effect of sediment granulometry on extraction efficiency of benthic meiofauna. The method uses colloidal silica Ludox((R)) (DuPont) HS 40 as a high density solution to extract meiofaunal organisms from sediment. Previous protocols using silica sols have had problems with physical interactions between the sediment and the specimens, causing large variations in extraction efficiencies between sediment types. The procedure presented herein reduces this problem by the addition of a 5 min period of fluidization of the sediment. The time used to complete the method is minimized with a final centrifugation step, which accelerates the settling rate of the fine sediment, resulting in a total time required for separation of approximately 20 min. Forty-eight samples were processed, with sediments ranging from sand to silty clay. The average extraction efficiency was 96.8 +/- 3.9% for the total meiofaunal abundance. Extraction efficiency was independent of sediment granulometry over the range of sediments tested.