GRAVES-DISEASE - PHENOTYPIC AND FUNCTIONAL-ANALYSIS AT THE CLONAL LEVEL OF THE T-CELL REPERTOIRE IN PERIPHERAL-BLOOD AND IN THYROID
GRAVES-DISEASE - PHENOTYPIC AND FUNCTIONAL-ANALYSIS AT THE CLONAL LEVEL OF THE T-CELL REPERTOIRE IN PERIPHERAL-BLOOD AND IN THYROID
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DOI：
10.1016/0090-1229(88)90075-x
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发表时间：
1988-05-01
期刊：
影响因子：
--
通讯作者：
CANONICA, GW
中科院分区：
文献类型：
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作者：
BAGNASCO, M;VENUTI, D;CANONICA, GW
我们使用高效克隆技术在克隆水平上研究了三名格雷夫斯病患者的甲状腺内和外周 T 淋巴细胞的所有组成部分。甲状腺内 T 细胞的克隆效率为 10% 至 31%，外周 T 细胞的克隆效率为 19% 至 100%。在格雷夫斯病中，表型分析显示甲状腺浸润物和外周血中 CD3+ CD4+ CD8- 和 CD3+ CD4- CD8+ 克隆的百分比相似。功能评估显示甲状腺浸润物中溶细胞克隆的比例与外周血相似或较低。此外，能够响应丝裂原刺激而释放白细胞介素2和/或γ-干扰素的甲状腺内和外周T细胞克隆的比例相似。最后，44%的甲状腺内克隆既不具有溶细胞性，也不能够释放IL-2和γ-干扰素。这些结果与在桥本氏甲状腺炎中获得的结果显着不同，在桥本氏甲状腺炎中，绝大多数甲状腺内T细胞克隆是溶细胞性的，并且与从外周血获得的结果相比，甲状腺浸润物中能够释放γ-IFN的克隆的比例显着增加。总而言之，这些数据表明 T 淋巴细胞在两种主要人类自身免疫性甲状腺疾病的发病机制中发挥着不同的作用。
We have investigated at the clonal level the repertoire of intrathyroid and peripheral T lymphocytes in three patients with Graves'' disease using a high efficiency cloning technique. Clonal efficiencies ranged from 10 to 31% for intrathyroid, and from 19 to 100% for peripheral T cells. In Graves'' disease the phenotypic analysis showed similar percentages of CD3+ CD4+ CD8- and CD3+ CD4- CD8+ clones in thyroid infiltrates and periheral blood. The functional evaluation showed similar or lower proportions of cytolytic clones in thyroid infiltrates with respect to peripheral blood. Furthermore, the proportions of intrathyroid and peripheral T-cell clones capable of releasing interleukin-2 and/or .gamma.-interferon in response to mitogen stimulation were similar. Finally, 44% of intrathyroid clones were neither cytolytic nor able to release IL-2 and .gamma.-interferon. These results are strikingly different from those obtained in Hashimoto''s thyroiditis, where the large majority of intrathyroid T-cell clones are cytolytic and the proportions of clones able to release .gamma.-IFN are remarkably increased in thyroid infiltrates when compared to those obtained from peripheral blood. Taken together, these data suggest a different role for T lymphocytes in the pathogenesis of the two major human autoimmune thyroid diseases.