ACUTE STRESS INDUCES TIME-DEPENDENT RESPONSES IN DOPAMINE MESOLIMBIC SYSTEM
ACUTE STRESS INDUCES TIME-DEPENDENT RESPONSES IN DOPAMINE MESOLIMBIC SYSTEM
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DOI：
10.1016/0006-8993(91)90192-x
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发表时间：
1991-07-19
期刊：
影响因子：
2.9
通讯作者：
CABIB, S
中科院分区：
文献类型：
--
作者：
PUGLISIALLEGRA, S;IMPERATO, A;CABIB, S
暴露于约束或足电击（3-60分钟）诱导类似的双相变化的3-甲氧基酪胺（3-MT）浓度（初始增加，然后降低到对照水平以下），在小鼠的隔丘核（NAS），如组织分析所示。 两种应激源之间的唯一区别是受约束小鼠的下降期开始得更早。 在这两种应激条件下，酸代谢产物，3，4-二羟基苯乙酸（DOPAC）和高香草酸（HVA）增加整个应力，而多巴胺（DA）浓度没有发生显着变化。 这些数据表明，双相变化的DA释放过程中长时间的应激暴露。 通过体内微透析（10-240 min）对受限清醒大鼠中的释放进行分析，结果显示NAS中存在类似的双相DA演变（初始增加，随后降低至基线水平以下）。 与先前实验的唯一差异是降低期延迟开始。 DOPAC和HVA的变化也很明显。 此外，释放的大鼠显示出DA释放超过基线水平的立即增加，也表明神经递质的消耗不能解释释放的DA的减少。这些结果支持了中脑边缘系统中DA传递的双相改变是对应激的一般反应的假设，并表明DA释放的初始增加代表唤醒反应，而随后的减少DA释放可能与应对失败有关。
Exposure to either restraint or footshock (3-60 min) induced similar biphasic alterations of 3-methoxytyramine (3-MT) concentrations (initial increase followed by decrease below control levels) in the nucleus accumbens septi (NAS) of mice, as revealed by tissue analysis. The only difference between the two stressors was the earlier onset of the decrease phase in the restrained mice. In both stressful conditions acid metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) increased throughout stress, while no significant changes in dopamine (DA) concentrations occurred. These data suggest biphasic alteration of DA release during prolonged stress exposure. The analysis of release in restrained conscious rats by in vivo microdialysis (10-240 min) showed a similar biphasic DA evolution (initial increase followed by decrease below baseline levels) in the NAS. The only difference from the previous experiment was the delayed onset of the decrease phase. Similar changes in DOPAC and HVA were also evident. Moreover, freed rats showed an immediate increase of DA release over baseline levels, also indicating that depletion of the neurotransmitter cannot account for the reduction of released DA. Taken together, these results support the hypothesis that biphasic alteration of DA transmission in the mesolimbic system is a general response to stress and suggest that the initial increase of DA release represents an arousal response while the subsequent decrease in DA release may be related to coping failure.