Fluorescence and Atomic Force Microscopy Imaging of Wall Teichoic Acids in Lactobacillus plantarum
Fluorescence and Atomic Force Microscopy Imaging of Wall Teichoic Acids in Lactobacillus plantarum
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DOI：
10.1021/cb1003509
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发表时间：
2011-04-01
影响因子：
4
通讯作者：
Dufrene, Yves F.
中科院分区：
文献类型：
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作者：
Andre, Guillaume;Deghorain, Marie;Dufrene, Yves F.
尽管磷壁酸是细菌细胞壁的主要成分，但对其空间定位与其功能作用之间的关系知之甚少。在这里，我们使用单分子原子力显微镜 (AFM) 与荧光显微镜相结合，对植物乳杆菌中壁磷壁酸 (WTA) 的分布及其生理作用进行成像。对野生型菌株和 WTA (Delta tagO) 或细胞壁多糖 (Delta cps1-4) 合成缺陷的突变菌株的表型分析表明，WTA 是适当的细胞伸长和细胞分裂所必需的。 AFM 纳米级成像表明，表达 WTA 的菌株具有高度极化的表面形态，极点比侧壁光滑得多。使用特定凝集素探针的 AFM 和荧光成像表明，偏振表面结构与 WTA 的异质分布相关，后者在两极表面不存在。这些观察结果表明，植物乳杆菌中 WTA 的极化分布在控制细胞形态发生（表面粗糙度、细胞形状、伸长和分裂）中起着关键作用。
Although teichoic acids are major constituents of bacterial cell walls, little is known about the relationships between their spatial localization and their functional roles. Here, we used single-molecule atomic force microscopy (AFM) combined with fluorescence microscopy to image the distribution of wall teichoic acids (WTAs) in. Lactobacillus plantarum, in relation with their physiological roles. Phenotype analysis of the wild-type strain and of mutant strains deficient for the synthesis of WTAs (Delta tagO) or cell wall polysaccharides (Delta cps1-4) revealed that WTAs are required for proper cell elongation and cell division. Nanoscale imaging by AFM showed that strains expressing WTAs have a highly polarized surface morphology, the poles being much smoother than the side walls. AFM and fluorescence imaging with specific lectin probes demonstrated that the polarized surface structure correlates with a heterogeneous distribution of WTAs, the latter being absent from the surface of the poles. These observations indicate that the polarized distribution of WTAs in L. plantarum plays a key role in controlling cell morphogenesis (surface roughness, cell shape, elongation, and division).