L-Arginine and L-Citrulline Supplementation Have Different Programming Effect on Regulatory T-Cells Function of Infantile Rats
L-Arginine and L-Citrulline Supplementation Have Different Programming Effect on Regulatory T-Cells Function of Infantile Rats
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DOI：
10.3389/fimmu.2018.02911
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发表时间：
2018-12-10
影响因子：
7.3
通讯作者：
Yu, Hong-Ren
中科院分区：
文献类型：
--
作者：
Lee, Yi-Chen;Su, Yu-Tsun;Yu, Hong-Ren
精氨酸是健康成人的半必需氨基酸，但对于早产儿、新生儿或危重患者是必需的。精氨酸可以从我们的饮食中提供，也可以从瓜氨酸中重新合成。在脓毒症或内毒素血症的情况下，精氨酸可能缺乏，并伴有免疫应答改变。补充L-精氨酸可以改善免疫失调状况，改善预后。许多研究尝试补充L-精氨酸或L-瓜氨酸，以检查对成年人免疫反应的影响。很少有人研究过这些小孩子。在这项研究中，我们确定了补充L-精氨酸和L-瓜氨酸对幼鼠免疫应答的影响。雄性幼鼠在出生后第8天至第14天腹腔注射生理盐水、L-精氨酸（200 mg/kg/天）或L-瓜氨酸（200 mg/kg/天）。然后处死幼鼠。在用刀豆球蛋白A（Con A）或脂多糖（LPS）刺激后，分析血液，同时指示脾脏用于免疫分析。我们发现L-精氨酸补充剂通过增加IFN-γ的产生来增强Th 1免疫应答。L-精氨酸和L-瓜氨酸治疗均可通过增加IL-10水平调节调节性T细胞（Treg）免疫效应。只有L-瓜氨酸组显示TGF-β 1增加。还注意到L-精氨酸和L-瓜氨酸治疗均降低SMAD 7表达并增强SIRT-1丰度。然而，FOXP 3的表达仅受L-瓜氨酸处理的调节。因此，我们得出结论，补充L-精氨酸和L-瓜氨酸可以不同地调节幼年大鼠的调节性T细胞功能。
Arginine is a semiessential amino acid in healthy adult human, but is essential for preterm, newborn or critically ill patients. Arginine can be supplied from our diet or de novo synthesis from citrulline. In conditions of sepsis or endotoxemia, arginine may be deficient and be accompanied with altered immune response. L-arginine supplementation can ameliorate dysregulated immune condition and improve prognosis. Many studies had tried L-arginine or L-citrulline supplementation to examine the effect on immune response in the adult population. Few had studied on the young children. In this study, we determined the effect of L-arginine and L-citrulline supplementation on the immune response of infantile rats. Male infantile rats received normal saline, L-arginine (200 mg/kg/day) or L-citrulline (200 mg/kg/day) intraperitoneally over postnatal day 8 to day 14. The infantile rats were then sacrificed. The blood was analyzed while the spleen was indicated for immune analysis after stimulation with concanavalin A (Con A) or lipopolysaccharide (LPS). We found L-arginine supplementation enhanced Th1 immune response by increasing IFN-gamma production. Both the L-arginine and L-citrulline therapy can modulate regulatory T-cell (Treg) immune effects by increasing the IL-10 level. Only the L-citrulline group showed a TGF-beta 1 increase. Both L-arginine and L-citrulline therapy were also noted to decrease SMAD7 expression and enhance SIRT-1 abundance. However, FOXP3 expression was only modulated by L-citrulline treatment. We then concluded that L-arginine and L-citrulline supplementation can modulate the regulatory T-cells function differently for infantile rats.