Changes in mRNA Expression of Nicotinic Acetylcholine Receptor Subunits during Embryonic Development of Mouse Masseter Muscle
Changes in mRNA Expression of Nicotinic Acetylcholine Receptor Subunits during Embryonic Development of Mouse Masseter Muscle
复制标题
小鼠咬肌胚胎发育过程中烟碱乙酰胆碱受体亚基mRNA表达的变化
作者：
A. Yamane;Takashi Saito;Y. Nakagawa;Y. Ohnuki;Y. Saeki
烟碱型乙酰胆碱受体（nAChR）从胚胎型（α2βγδ亚基）转变为成人型（α2βεδ亚基），并随着躯干和四肢骨骼肌的发育而在神经肌肉接头处消失。然而，人们对咬肌胚胎发育过程中的这一过程知之甚少。为了确定小鼠咬肌中 nAChR 从胚胎 (E) 11 天到新生儿阶段转变的时间过程，我们通过竞争性聚合酶链反应结合逆转录分析了 δ、ε 和 γ 亚基 mRNA 的表达水平，并通过免疫组织化学分析了 δ 亚基蛋白的分布。 nAChR δ 亚基 mRNA 最初在 E11 时检测到，在 E11 和 E17 之间显示出约 25 倍的增加 (p<0.0001)，此后趋于稳定，直到新生儿阶段。在 E17 和出生时在整个咬肌肌纤维中观察到 δ 亚基的免疫染色，表明 nAChR 消除甚至在新生儿阶段也没有开始。 ε亚基mRNA最初出现在E17，直到新生儿阶段数量增加了144% (p<0.0001)。在E11和E17之间，γ亚基mRNA的数量增加了约240%（p<0.0001），然后在新生儿阶段比E17值减少了22%（p<0.05）。 ε亚基mRNA表达的开始与γ亚基mRNA数量减少的开始一致，表明nAChR亚基转换从E17开始。
Nicotinic acetylcholine receptors (nAChRs) switch from the embryonic-type (α2βγδ subunits) to the adult-type (α2βεδ subunits), and disappear besides the neuromuscular junctions with the development of trunk and limb skeletal muscles. However, little is known about this process during the embryonic development of masseter muscle. To identify the time course of the nAChR transition from embryonic day (E) 11 to the newborn stage in mouse masseter muscle, we analyzed the expression level of δ, ε, and γ subunit mRNAs by competitive polymerase chain reaction in combination with reverse transcription as well as distribution of δ subunit protein by immunohistochemistry. The nAChR δ subunit mRNA was initially detected at E11, showed an approximately 25-fold increase (p<0.0001) between E11 and E17, and plateaued thereafter until the newborn stage. Immunostaining for δ subunit was observed in the whole portions of masseter myofibers at E17 and birth, suggesting that the nAChR elimination does not begin even at the newborn stage. The ε subunit mRNA initially appeared at E17, and increased in quantity by 144% (p<0.0001) up to the newborn stage. The quantity of γ subunit mRNA increased by approximately 240% (p<0.0001) between E11 and E17, and then decreased by 22% (p<0.05) from E17 value at the newborn stage. The beginning of the expression of the ε subunit mRNA was coincident with the beginning of the decrease in the quantity of the γ subunit mRNA, suggesting that the nAChR subunit switch begins at E17.
DOI：
10.1073/pnas.87.7.2725
发表时间：
1990-04-01
影响因子：
11.1
作者：
GILLILAND, G;PERRIN, S;BUNN, HF
通讯作者：
BUNN, HF
在小鼠胚胎舌头发育的成肌细胞分化过程中诱导 myoD、肌细胞生成素和结蛋白的表达。
影响因子：
3
作者：
Yamane,A;Takahashi,K;Mayo,M;Vo,H;Shum,L;Zeichner-David,M;Slavkin,HC
通讯作者：
Slavkin,HC
人类胚胎、新生儿和慢骨骼肌球蛋白重链的异构体特异性 cDNA。
影响因子：
5.2
作者：
Stedman,HH;Kelly,AM;Rubinstein,NA
通讯作者：
Rubinstein,NA