Distribution of heme oxygenase-2 in nerves and interstitial cells of Cajal in the normal pylorus and in infantile hypertrophic pyloric stenosis.
Distribution of heme oxygenase-2 in nerves and interstitial cells of Cajal in the normal pylorus and in infantile hypertrophic pyloric stenosis.
复制标题
血红素加氧酶-2 在正常幽门和婴儿肥厚性幽门狭窄的神经和 Cajal 间质细胞中的分布。
DOI：
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发表时间：
2003
影响因子：
4.6
通讯作者：
P. Puri
中科院分区：
文献类型：
--
作者：
A. Piotrowska;V. Solari;P. Puri
CONTEXT
Interstitial cells of Cajal (ICCs) are pacemaker cells, which are of fundamental importance in regulating gastrointestinal motility. Recent evidence suggests that carbon monoxide is a neurotransmitter involved in neurotransmission between ICC and smooth muscle cells. Heme oxygenase-2 (HO-2) is the major physiological mechanism for the generation of carbon monoxide in the enteric nervous system.
OBJECTIVE
To investigate the immunocolocalization of HO-2 and ICCs in the normal pylorus and in infantile hypertrophic pyloric stenosis (IHPS).
DESIGN
Specimens from 18 infants with IHPS and 8 control specimens were examined using double-immunostaining with c-Kit and HO-2 antibodies. The immunolocalization was detected with the help of confocal laser scanning microscopy.
RESULTS
Abundant HO-2 immunoreactivity was found in ICCs in the smooth muscle layer of normal pylorus. There was a decrease in the number of ICCs and lack of HO-2 immunoreactivity in ICCs in IHPS.
CONCLUSIONS
The results of the present study provide the first evidence for the presence of HO-2 in ICCs in the normal human pylorus. The lack of ICCs and HO-2 in IHPS suggests impaired intracellular communication between ICCs and smooth muscle cells, contributing to motility dysfunction in IHPS.
DOI：
10.1152/ajpgi.1993.264.6.g1184
发表时间：
1993-06
期刊：
The American journal of physiology
影响因子：
--
作者：
G. Farrugia;W. A. Irons;J. Rae;Michel G. Sarr;J. Szurszewski
通讯作者：
G. Farrugia;W. A. Irons;J. Rae;Michel G. Sarr;J. Szurszewski
影响因子：
29.4
作者：
Ward, SM;Morris, G;Sanders, KM
通讯作者：
Sanders, KM