Transgenic animals: current and alternative strategies.
Transgenic animals: current and alternative strategies.
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DOI：
10.1089/15204559950020076
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发表时间：
1999-01-01
期刊：
影响因子：
--
通讯作者：
Chan, A W
中科院分区：
文献类型：
--
作者：
Chan, A W
转基因动物技术是近二十年来发展起来的最具吸引力的技术之一。它使我们能够解决生命科学中其他方法无法解决的问题。对生物医学和生物学研究以及商业利益的影响是压倒性的。伴随着这种快速发展的技术及其多样化应用的问题吸引了各种实体的关注。尽管如此，仍然存在的最基本的问题之一是寻找一种有效和可靠的基因传递系统来创造转基因动物。传统的原核显微注射方法在不同物种间的成功率差异很大。虽然在转基因小鼠的生产中已经获得了可接受的效率，但是相对低的效率（
Transgenic animal technology is one of the most fascinating technologies developed in the last two decades. It allows us to address questions in life sciences that no other methods have achieved. The impact on biomedical and biological research, as well as commercial interests are overwhelming. The questions accompanying this fast growing technology and its diversified applications attract the attention from a variety of entities. Still, one of the most fundamental problems remaining is the search for an efficient and reliable gene delivery system for creating transgenic animals. The traditional method of pronuclear microinjection has displayed great variability in success among species. While an acceptable efficiency in the production of transgenic mice has been attained, the relative low efficiency (