Association of DNA-dependent and -independent ribonucleoside triphosphatase activities with dnaB gene product of Escherichia coli.
Association of DNA-dependent and -independent ribonucleoside triphosphatase activities with dnaB gene product of Escherichia coli.
复制标题
DNA依赖性和非依赖性核糖核苷三磷酸酶活性与大肠杆菌的dnaB基因产物的关联。
DOI：
10.1073/pnas.71.3.783
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发表时间：
1974
影响因子：
11.1
通讯作者：
J. Hurwitz
中科院分区：
文献类型：
--
作者：
S. Wickner;M. Wright;J. Hurwitz
大肠杆菌 dnaB 基因产物的制剂含有核糖核苷三磷酸酶活性，可被 DNA 刺激 10 倍。三磷酸酶活性的产物是核苷二磷酸和P(i)。 varphiX174 DNA 依赖性系统中的 dnaB 补充活性和这些三磷酸酶活性在最后 20 倍的广泛（约 40,000 倍）纯化过程中进行共纯化。纯化材料的丙烯酰胺凝胶电泳显示出与洗脱的 dnaB 互补以及 DNA 依赖性和非依赖性核苷三磷酸酶活性一致的蛋白质单条带。
Preparations of E. coli dnaB gene product contain ribonucleoside triphosphatase activity that is stimulated 10-fold by DNA. The products of the triphosphatase activity are nucleoside diphosphates and P(i). The dnaB complementing activity in the varphiX174 DNA-dependent system and these triphosphatase activities copurify over the last 20-fold of an extensive (about 40,000-fold) purification procedure. Acrylamide gel electrophoresis of the purified material shows a single band of protein coincident with eluted dnaB complementing and DNA-dependent and -independent nucleoside triphosphatase activities.