Changes in the rat heart proteome induced by exercise training: Increased abundance of heat shock protein hsp20
Changes in the rat heart proteome induced by exercise training: Increased abundance of heat shock protein hsp20
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DOI：
10.1002/pmic.200401356
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发表时间：
2006-05-01
期刊：
影响因子：
3.4
通讯作者：
Li, Zhao Bo
中科院分区：
文献类型：
--
作者：
Boluyt, Marvin O.;Brevick, Julie L.;Li, Zhao Bo
长期的运动训练可以增强心脏的适应性，改善泵功能，并提供心脏保护。为了确定运动训练刺激这种有利表型的分子机制，采用蛋白质组学方法检测运动训练后差异表达或修饰的大鼠心脏蛋白。运动训练的大鼠进行了为期六周的渐进式跑步机训练，每周五天，0%等级，使用间隔训练协议。久坐不动的对照组大鼠的年龄和体重与运动训练组大鼠相匹配。在最后一次运动后的不同时间（0-72小时）收获心脏，并用于生成2-D电泳蛋白质组图谱和免疫印迹。与安静对照组相比，运动性心肌肥厚组心肌中有26个蛋白质斑点强度发生显著变化（p < 0.05），其中12个蛋白质斑点仅出现在运动性心肌凝胶上。免疫印迹证实，慢性运动训练，但不是一个单一的回合的运动，引起了类似的2.5倍的增加，在心脏中的一个候选蛋白的丰度，类似于20 kDa的热休克蛋白（hsp 20），持续至少72小时的停训。因此，运动训练改变了大鼠心脏的心脏蛋白质组;这些变化包括热休克蛋白20表达的显着增加。
Chronic exercise training elicits adaptations in the heart that improve pump function and confer cardioprotection. To identify molecular mechanisms by which exercise training stimulates this favorable phenotype, a proteomic approach was employed to detect rat cardiac proteins that were differentially expressed or modified after exercise training. Exercise-trained rats underwent six weeks of progressive treadmill training five days/week, 0% grade, using an interval training protocol. Sedentary control rats were age- and weight-matched to the exercise-trained rats. Hearts were harvested at various times (0-72 h) after the last bout of exercise and were used to generate 2-D electrophoretic proteome maps and immunoblots. Compared with hearts of sedentary rats, 26 protein spot intensities were significantly altered in hypertrophied hearts of exercise-trained rats (p < 0.05), and 12 spots appeared exclusively on gels from hearts of exercise-trained rats. Immunoblotting confirmed that chronic exercise training, but not a single bout of exercise, elicited a similar to 2.5-fold increase in the abundance of one of the candidate proteins in the heart, a similar to 20 kDa heat shock protein (hsp20) that persisted for at least 72 h of detraining. Thus, exercise training alters the cardiac proteome of the rat heart; the changes include a marked increase in the expression of hsp20.