Bioconjugation of Functionalized Oligodeoxynucleotides with Fluorescence Reporters for Nanoparticle Assembly.
Bioconjugation of Functionalized Oligodeoxynucleotides with Fluorescence Reporters for Nanoparticle Assembly.
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功能化寡脱氧核苷酸与荧光报告基因的生物共轭用于纳米颗粒组装。
DOI：
10.1007/978-1-0716-3417-2_6
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Khisamutdinov,EmilF
中科院分区：
文献类型：
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作者：
Doe,Erwin;Hayth,HannahL;Khisamutdinov,EmilF
在核酸纳米技术和治疗学领域中，迫切需要通过寡核苷酸结构的化学修饰或将它们共价连接到具有生物学相关性质的报告分子或治疗部分来改善寡脱氧核苷酸（ODN）的性质。因此，化学缀合不仅可以显着改善ODN的固有性质，而且还可以改善报告/治疗分子的固有性质。核酸与小分子的生物缀合也用作纳米递送设施以将各种功能性运输至特定靶标。在此，我们描述了一种通用的方法，其部署叠氮化物-炔环加成，点击反应以将花青-3炔部分缀合至叠氮化物官能化的ODN 12-mer，以及3-叠氮基7-羟基香豆素缀合至炔官能化的ODN 12-mer。
In the field of nucleic acid nanotechnology and therapeutics, there is an imperative need to improve the oligodeoxynucleotides’ (ODNs) properties by either chemical modification of the oligonucleotides’ structure or to covalently link them to a reporter or therapeutic moieties that possess biologically relevant properties. The chemical conjugation can thus significantly improve the intrinsic properties not only of ODNs but also reporter/therapeutic molecules. Bioconjugation of nucleic acids to small molecules also serves as a nano-delivery facility to transport various functionalities to specific targets. Herein, we describe a generalized methodology that deploys azide-alkyne cycloaddition, a click reaction to conjugate a cyanine-3 alkyne moiety to an azide-functionalized ODN 12-mer, as well as 3-azido 7-hydroxycoumarin to an alkyne functionalized ODN 12-mer.