Subcellular sequencing of single neurons reveals the dendritic transcriptome of GABAergic interneurons.
Subcellular sequencing of single neurons reveals the dendritic transcriptome of GABAergic interneurons.
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DOI：
10.7554/elife.63092
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发表时间：
2021-01-06
期刊：
影响因子：
7.7
通讯作者：
Schuman EM
中科院分区：
文献类型：
--
作者：
Perez JD;Dieck ST;Alvarez-Castelao B;Tushev G;Chan IC;Schuman EM
Although mRNAs are localized in the processes of excitatory neurons, it is still unclear whether interneurons also localize a large population of mRNAs. In addition, the variability in the localized mRNA population within and between cell types is unknown. Here we describe the unbiased transcriptomic characterization of the subcellular compartments of hundreds of single neurons. We separately profiled the dendritic and somatic transcriptomes of individual rat hippocampal neurons and investigated mRNA abundances in the soma and dendrites of single glutamatergic and GABAergic neurons. We found that, like their excitatory counterparts, interneurons contain a rich repertoire of ~4000 mRNAs. We observed more cell type-specific features among somatic transcriptomes than their associated dendritic transcriptomes. Finally, using celltype-specific metabolic labeling of isolated neurites, we demonstrated that the processes of glutamatergic and, notably, GABAergic neurons were capable of local translation, suggesting mRNA localization and local translation are general properties of neurons.
