MicroRNA-21 down-regulates the expression of tumor suppressor PDCD4 in human glioblastoma cell T98G
MicroRNA-21 down-regulates the expression of tumor suppressor PDCD4 in human glioblastoma cell T98G
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MicroRNA-21下调人胶质母细胞瘤细胞T98G中肿瘤抑制因子PDCD4的表达
DOI：
10.1016/j.canlet.2008.06.034
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发表时间：
2008-12-18
期刊：
影响因子：
9.7
通讯作者：
Peng, Xiaozhong
中科院分区：
文献类型：
--
作者：
Chen, Yang;Liu, Wei;Peng, Xiaozhong
微小RNA（MicroRNAs）与不同的癌症相关过程有关。微小RNA miR - 21在胶质母细胞瘤中似乎起到抗凋亡因子的作用。然而，在胶质母细胞瘤中，miR - 21的功能性靶基因在很大程度上是未知的。在这项研究中，利用生物信息学分析来确定各种基因中的miR - 21靶位点。荧光素酶活性测定表明，一些参与凋亡的基因，如PDCD4、MTAP和SOX5，带有假定的miR - 21结合位点。在许多人类胶质母细胞瘤细胞系（如T98G、A172、U87和U251）中，PDCD4蛋白的表达与miR - 21的表达呈负相关。抑制miR - 21会增加T98G细胞系中内源性PDCD4的水平，而过表达miR - 21会抑制PDCD4依赖性凋亡。总之，这些结果表明，miR - 21的表达在调节胶质母细胞瘤的细胞过程中起着关键作用，并可能成为有效治疗的靶点。（c）2008爱思唯尔爱尔兰有限公司。保留所有权利。
MicroRNAs have been linked to different cancer-related processes. The microRNA miR-21 appears to function as an anti-apoptosis factor in glioblastomas. However, the functional target genes of miR-21 are largely unknown in glioblastomas. In this study, bioinformatics analysis was used to identify miR-21 target sites in various genes. Luciferase activity assay showed that a number of genes involved in apoptosis, PDCD4, MTAP, and SOX5, carry putative miR-21 binding sites. Expression of PDCD4 protein correlates inversely with expression of miR-21 in a number of human glioblastoma cell lines such as T98G, A172, U87, and U251. Inhibition of miR-21 increases endogenous levels of PDCD4 in cell line T98G and over-expression miR-21 inhibits PDCD4-dependent apoptosis. Together, these results indicate that miR-21 expression plays a key role in regulating cellular processes in glioblastomas and may serve as a target for effective therapies. (c) 2008 Elsevier Ireland Ltd. All rights reserved.