A formula for thermal stability (Tm) prediction of PNA/DNA duplexes
A formula for thermal stability (Tm) prediction of PNA/DNA duplexes
复制标题
DOI：
10.1093/nar/26.21.5004
复制
发表时间：
1998-11-01
影响因子：
14.9
通讯作者：
Nielsen, PE
中科院分区：
文献类型：
--
作者：
Giesen, U;Kleider, W;Nielsen, PE
推导了预测PNA/DNA双链体热稳定性的经验公式。该模型是基于T-m计算的相应的DNA/DNA双链体采用最近邻的方法，包括嘧啶含量和长度的PNA考虑到PNA/DNA杂交体的热稳定性增加和PNA-DNA异源双链体的不对称性的条款。T-m预测公式的预测能力受到未用于模型构建的独立数据集的挑战。>90%的序列的T-m预测在5 K以内; 98%的预测T（m）s与实验测定的T-m相差不超过10 K。
An empirical formula for thermal stability (T-m) prediction of PNA/DNA duplexes has been derived. The model is based on the T-m as calculated for the corresponding DNA/DNA duplex employing a nearest neighbour approach, by including terms for the pyrimidine content and length of the PNA to take into account the increased thermostability of PNA/DNA hybrids and the asymmetry of the PNA-DNA heteroduplex. The predictive power of the T-m prediction formula was challenged with an independent data set not used for model building. The T-m of >90% of the sequences was predicted within 5 K; 98% of the predicted T(m)s differ by not more than 10 K from the experimentally determined T-m.