Redox balance, metabolic fingerprint and physiological characterization in contrasting North East Indian rice for Aluminum stress tolerance
Redox balance, metabolic fingerprint and physiological characterization in contrasting North East Indian rice for Aluminum stress tolerance
复制标题
DOI：
10.1038/s41598-019-45158-3
复制
发表时间：
2019-06-18
影响因子：
4.6
通讯作者：
Panda, Sanjib Kumar
中科院分区：
文献类型：
--
作者：
Awasthi, Jay Prakash;Saha, Bedabrata;Panda, Sanjib Kumar
铝毒是影响酸性土壤水稻生产的重要问题。本工作进行了寻找改变活性氧体内平衡，代谢指纹，和形态在两个对比籼稻品种的东北印度（印度东北部）铝毒性。铝胁迫导致ROS（H2 O2和O-2（-））的过量积累，这反过来又诱导ROS介导的细胞损伤，如定性和定量的脂质过氧化所示。这种过量的ROS产生也导致叶绿素含量和气孔的显着减少！电导这是其次是由叶绿素荧光检测光合效率的损失。ROS造成的这种过度损伤促使我们检查抗氧化机制。抗氧化剂，尤其是酶（SOD、APX、PDX、GR、CAT、达尔、MDHAR）在维持活性氧稳态方面发挥着非常重要的作用。耐铝品种地桑的这些酶活性高于敏感品种，敏感品种则相反。非酶抗氧化剂（脯氨酸，抗坏血酸和谷胱甘肽）也表现出类似的趋势。虽然耐受性品种显示出较强的抗氧化机制，但它不能完全消除幼苗所经历的胁迫。有机酸也是根际铝胁迫解毒的重要因素。在耐受基因型，柠檬酸渗出物被发现是更多的相比，敏感基因型暴露于高剂量的铝。这是由较高丰度的FRDL 4，柠檬酸转运蛋白支持。不仅FRDL 4，Al胁迫反应的其他利益相关者如ART 1和ALS 1在耐受品种中描绘了显著的转录本丰度。总之，通过这项研究，详细的生理和代谢特性的两个对比籼稻品种迪桑和Joymati，原产于印度东北部的铝耐受性进行了第一次。
Aluminum (Al) toxicity is a serious problem for rice crop productivity in acidic soils worldwide. The present work was conducted to look out for the alteration in ROS homeostasis; metabolic fingerprint; and morphology in two contrasting Indica rice cultivars of North East India (NE India) to Al toxicity. Al stress led to excess accumulation of ROS (H2O2 and O-2(-)), and this in turn induced ROS mediated cellular damage, as indicated by lipid peroxidation both qualitatively as well as quantitatively. This excessive ROS production also led to significant reduction in chlorophyll content and stomata! conductance. This was followed by the loss of photosynthetic efficiency as detected by chlorophyll fluorescence. This excessive damage due to ROS prompted us to check the anti-oxidative machinery. Antioxidants, especially enzymes (SOD, APX, PDX, GR, CAT, DHAR, MDHAR) are very important players in maintenance of ROS homeostasis. In tolerant variety Disang, higher activity of these enzymes and vice versa in sensitive variety, was observed in response to Al treatment. The non-enzymatic antioxidants (proline, ascorbate and glutathione) also showed similar trend. Though the tolerant variety showed strong anti-oxidative machinery, it was unable to completely nullify the stress experienced by the seedlings. Organic acids are also important players in detoxification of Al stress through efflux in the rhizosphere. In tolerant genotype, citrate exudate was found to be more when compared to sensitive genotypes on exposure to high dose of Al. This is supported by higher abundance of FRDL4, a citrate transporter. Not only FRDL4, other stakeholders for Al stress response like ART1 and ALS1depicted prominent transcript abundance in the tolerant variety. In conclusion, through this study detailed physiological and metabolic characterisation of two contrasting Indica rice varieties Disang and Joymati, native to NE India for Al tolerance was performed for the very first time.