Diversification of the cullin family.
Diversification of the cullin family.
复制标题
DOI：
10.1186/1471-2148-9-267
复制
发表时间：
2009-11-19
影响因子：
3.4
通讯作者：
Marín I
中科院分区：
文献类型：
--
作者：
Marín I
Cullins 是通过参与多亚基泛素连接酶复合物参与泛素化的蛋白质。在这项研究中，我使用比较基因组数据来建立真核生物中库林的出现和多样化模式。现有数据表明，在unikont/bikont分裂之前存在三个cullin基因，我将其称为Culα、Culβ和Culγ。真菌物种相当严格地保守这三个祖先基因，仅偶尔出现谱系特异性重复。相反，在动物或植物谱系中出现了一些额外的基因。例如，人类基因Cul1、Cul2、Cul5、Cul7和Parc均源自祖先Culα基因。这些结果与现有的功能数据一起表明，早期真核进化中已经存在三种不同类型的含有泛素连接酶cullin的复合物：1）与Culα蛋白的SCF样复合物； 2) Culβ/BTB复合物； 3) 含有 Culγ 和 DDB1 样蛋白的复合物。含有延长蛋白的复合物最近在动物和真菌中出现，可能两次独立出现。大多数已知类型的含有 cullin 的泛素连接酶复合物都是古老的。现有数据表明，自真核生物起源以来，复合体多样性主要是通过紧密相关的亚基组合产生的，而产生新型复合体的激进创新却很少。然而，迄今为止尚未研究的几个原生生物群含有高度分化的库林，表明可能存在其他类型的复合体。
Cullins are proteins involved in ubiquitination through their participation in multisubunit ubiquitin ligase complexes. In this study, I use comparative genomic data to establish the pattern of emergence and diversification of cullins in eukaryotes. The available data indicate that there were three cullin genes before the unikont/bikont split, which I have called Culα, Culβ and Culγ. Fungal species have quite strictly conserved these three ancestral genes, with only occasional lineage-specific duplications. On the contrary, several additional genes appeared in the animal or plant lineages. For example, the human genes Cul1, Cul2, Cul5, Cul7 and Parc all derive from the ancestral Culα gene. These results, together with the available functional data, suggest that three different types of ubiquitin ligase cullin-containing complexes were already present in early eukaryotic evolution: 1) SCF-like complexes with Culα proteins; 2) Culβ/BTB complexes; and, 3) Complexes containing Culγ and DDB1-like proteins. Complexes containing elongins have arisen more recently and perhaps twice independently in animals and fungi. Most of the known types of cullin-containing ubiquitin ligase complexes are ancient. The available data suggest that, since the origin of eukaryotes, complex diversity has been mostly generated by combining closely related subunits, while radical innovations, giving rise to novel types of complexes, have been scarce. However, several protist groups not examined so far contain highly divergent cullins, indicating that additional types of complexes may exist.
登录
查看更多内容
影响因子：
56.9
作者：
Kamura, T;Koepp, DM;Conway, JW
通讯作者：
Conway, JW
影响因子：
7.2
作者：
Dieterle, M;Thomann, A;Genschik, P
通讯作者：
Genschik, P
影响因子：
64.5
作者：
Feldman, RMR;Correll, CC;Deshaies, RJ
通讯作者：
Deshaies, RJ
影响因子：
2.1
作者：
Kominami, K;Ochotorena, I;Toda, T
通讯作者：
Toda, T
影响因子：
30.8
作者：
Huber, C;Dias-Santagata, D;Cormier-Daire, V
通讯作者：
Cormier-Daire, V