GAS CHROMATOGRAPHIC–MASS SPECTROMETRIC ANALYSIS OF BIOGENIC AMINES IN IDENTIFIED NEURONS AND TISSUES OF HIRUDO MEDICINALIS
GAS CHROMATOGRAPHIC–MASS SPECTROMETRIC ANALYSIS OF BIOGENIC AMINES IN IDENTIFIED NEURONS AND TISSUES OF HIRUDO MEDICINALIS
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已鉴定的药用水蛭神经元和组织中生物胺的气相色谱-质谱分析
DOI：
10.1111/j.1471-4159.1976.tb04452.x
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发表时间：
1976
影响因子：
4.7
通讯作者：
R. Coggeshall
中科院分区：
文献类型：
--
作者：
D. McAdoo;R. Coggeshall
- 以前的工作者报告说，药用水蛭节段神经节中的Retzius巨大细胞含有约2.3 pmol的5-羟色胺（5-HT）/细胞体。我们通过气相色谱-质谱法验证了Retzius细胞中5-HT的鉴定，并通过分析8个单独解剖的Retzius细胞体得出1.3-4.1 pmol/神经元的浓度。Retzius细胞体在每个神经节中含有约30%的5-HT。在水蛭神经系统周围的纤维性色素组织中发现平均25 pmol 5-HT/mg组织，浓度比Retzius细胞低约500倍。我们在Retzius细胞、色素组织或整个神经节中（多巴胺（±0.4 pmol/神经节）可能除外）均未检测到γ-氨基丁酸、章鱼胺、多巴胺或去甲肾上腺素。此外，我们无法在100个非嗜铬对照神经元的合并样本中检测到5-HT。
—Previous workers have reported that the colossal cells of Retzius in the segmental ganglia of the medicinal leech contain about 2.3 pmol of 5‐hydroxytryptamme (5‐HT)/cell body. We verify the identify of 5‐HT in the Retzius cells by gas chromatography‐mass spectrometry and derive concentrations of 1.3–4.1 pmol/neuron by analyses of eight individually dissected Retzius cell bodies. The Retzius cell bodies contain about 30% of the 5‐HT in each ganglion. An average of 25 pmol 5‐HT/mg tissue, a concentration about 500 times lower than that in the Retzius cell, was found in the fibrous, pigmented tissue surrounding the leech nervous system. We could not detect γ‐aminobutyric acid, octopamine, dopamine or norepinephrine in the Retzius cells, in the pigmented tissue, or, with the possible exception of dopamine (±0.4 pmol/ganglion), in whole ganglia. Furthermore, we could not detect 5‐HT in pooled samples of 100 non‐chromaffin control neurons.