Synthetic ePTFE Grafts Coated with an Anti-CD133 Antibody-Functionalized Heparin/Collagen Multilayer with Rapid in vivo Endothelialization Properties
Synthetic ePTFE Grafts Coated with an Anti-CD133 Antibody-Functionalized Heparin/Collagen Multilayer with Rapid in vivo Endothelialization Properties
复制标题
涂有抗 CD133 抗体功能化肝素/胶原多层且具有快速体内内皮化特性的合成 ePTFE 移植物
DOI：
10.1021/am401706w
复制
发表时间：
2013-08-14
影响因子：
9.5
通讯作者：
Wang, Chunsheng
中科院分区：
文献类型：
--
作者：
Lu, Shuyang;Zhang, Peng;Wang, Chunsheng
一种由肝素/胶原蛋白功能化的抗cd133抗体在扩展的聚四氟乙烯（ePTFE）移植物上被开发，以加速早期内皮化。ePTFE移植物的表面修饰表明，该多层膜在静态孵育和振动条件下是稳定的，并且抗cd133抗体成功交联到表面。血液相容性试验显示，无论是否存在抗cd133抗体，共固定肝素/胶原膜均可延长凝血时间，血小板活化和聚集较少，溶血率与裸ePTFE相当。CD133抗体包被的肝素/胶原合成血管移植物细胞毒性较小，细胞增殖不受抑制。在细胞粘附试验中，内皮细胞与改性ePTFE移植物粘附良好。采用猪颈动脉移植模型对改良ePTFE移植物进行体内评价。组织病理学染色和扫描电镜结果显示，抗cd133抗体能够加速血管内皮细胞附着在ePTFE移植物上，导致早期快速内皮化。抗cd133抗体功能化肝素/胶原多层膜的成功将为人工血管移植物的表面修饰提供一个有效的选择体系，提高其在临床中的应用。
An anti-CD133 antibody multilayer functionalized by heparin/collagen on an expanded polytetrafluoroethylene (ePTFE) graft was developed to accelerate early endothelializaton. The surface modification of ePTFE grafts demonstrated that the multilayer is stable in static incubation and shaking conditions and that the anti-CD133 antibodies were successfully cross-linked onto the surface. Blood compatibility tests revealed that the coimmobilized heparin/collagen films in the presence or absence of anti-CD133 antibodies prolonged the blood coagulation time and that there was less platelet activation and aggregation, whereas the hemolysis rate was comparable with the bare ePTFE grafts. Cellular proliferation was not inhibited, as the heparin/collagen synthetic vascular grafts coated with CD133 antibody showed little cytotoxicity. The endothelial cells adhered well to the modified ePTFE grafts during a cell adhesion assay. A porcine carotid artery transplantation model was used to evaluate the modified ePTFE grafts in vivo. The results of histopathological staining and scanning electron microscopy indicated that the anti-CD133 antibody was able to accelerate the attachment of vascular endothelial cells onto the ePTFE grafts, resulting in early rapid endothelialization. The success of the anti-CD133 antibody-functionalized heparin/collagen multilayer will provide an effective selection system for the surface modification of synthetic vascular grafts and improve their use in clinical applications.