Long-term survival and functional maintenance of hepatocytes by using a microfabricated cell array
Long-term survival and functional maintenance of hepatocytes by using a microfabricated cell array
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DOI：
10.1016/j.colsurfb.2012.04.022
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发表时间：
2012-09-01
影响因子：
5.8
通讯作者：
Nagasaki, Yukio
中科院分区：
文献类型：
--
作者：
Ikeda, Yutaka;Jomura, Tomoko;Nagasaki, Yukio
我们开发了一种微制造的肝细胞球体细胞阵列，显示出长期的活力，并保留了亲本肝细胞的特性。将从8周龄Wistar大鼠收获的新鲜肝细胞与饲养细胞共培养以快速形成肝细胞球状体;这些细胞保持球状体形成42天。我们还评估了肝细胞的细胞功能，如白蛋白分泌和细胞色素P450（CYP）的代谢活性。在肝细胞与饲养细胞共培养的球状体中，这些细胞功能甚至在42天后仍保留。因此，这种新型的共培养物将是非常有用的，不仅对肝脏疾病的机制和治疗的研究，而且对肝细胞毒性的早期预测在药物开发的临床前阶段。(C)2012 Elsevier B. V.保留所有权利。
We developed a microfabricated cell array of hepatocyte spheroids that showed long-term viability and retained the properties of the parent hepatocytes. Fresh hepatocytes harvested from 8-week-old Wistar rats were cocultured with feeder cells to rapidly form hepatocyte spheroids; these cells retained the spheroidal formation for 42 days. We also evaluated the cellular functions of the hepatocytes such as albumin secretion and metabolic activity of cytochrome P450 (CYP). In spheroids in which hepatocytes were cocultured with feeder cells, these cellular functions were retained even after 42 days. Therefore, this novel coculture will be very useful not only for research on the mechanism and treatment of liver diseases but also for early prediction of hepatocyte toxicity in the pre-clinical phase of drug development. (C) 2012 Elsevier B.V. All rights reserved.