Ultra Performance Liquid Chromatography-Atmospheric Pressure Photoionization-Tandem Mass Spectrometry for High-Sensitivity and High-Throughput Analysis of US Environmental Protection Agency 16 Priority Pollutants Polynuclear Aromatic Hydrocarbons
Ultra Performance Liquid Chromatography-Atmospheric Pressure Photoionization-Tandem Mass Spectrometry for High-Sensitivity and High-Throughput Analysis of US Environmental Protection Agency 16 Priority Pollutants Polynuclear Aromatic Hydrocarbons
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DOI：
10.1021/ac802275e
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发表时间：
2009-03-15
影响因子：
7.4
通讯作者：
Balogh, Michael P.
中科院分区：
文献类型：
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作者：
Cai, Sheng-Suan;Syage, Jack A.;Balogh, Michael P.
在这项工作中，我们展示了超高效液相色谱-大气压光电离-串联质谱 (UPLC-APPI-MS/MS) 对美国环境保护署 (U.S. EPA) 16 种重点污染物多环芳烃 (PAH) 进行高灵敏度和高通量分析的实用性。分析是在配备 Syagen PhotoMate APPI 源的 Waters Acquity-TQD 上进行的。所有 16 种多环芳烃在大约 3.5 分钟内即可在色谱柱上进行分析，所有多环芳烃异构体均具有出色的色谱分离效果，并且使用氯苯作为掺杂剂，所有分析物的色谱柱检测限均较低。经过评估，发现动态线性范围覆盖至少 3-4 个数量级。与现有的美国 EPA 方法相比，该方法将仪器样品通量提高了至少 10 倍。
In this work, we demonstrate the utility of ultra performance liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry (UPLC-APPI-MS/MS) for high-sensitivity and high-throughput analysis of United States Environmental Protection Agency (U.S. EPA) 16 priority pollutants polycyclic aromatic hydrocarbons (PAHs). Analyses were performed on a Waters Acquity-TQD equipped with Syagen's PhotoMate APPI source. All 16 PAHs were analyzed on column in approximately 3.5 min with excellent chromatographic separation for all PAH isomers and with low picogram detection limits on column for all analytes using chlorobenzene as a dopant. Dynamic linear ranges were evaluated and found to cover at least 3-4 orders of magnitude. In comparison with the existing U.S. EPA methods, this approach improves instrument sample throughput by at least 10-fold.