Persistent exposure to Mycoplasma induces malignant transformation of human prostate cells.
Persistent exposure to Mycoplasma induces malignant transformation of human prostate cells.
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DOI：
10.1371/journal.pone.0006872
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发表时间：
2009-09-01
期刊：
影响因子：
3.7
通讯作者：
Rosser CJ
中科院分区：
文献类型：
--
作者：
Namiki K;Goodison S;Porvasnik S;Allan RW;Iczkowski KA;Urbanek C;Reyes L;Sakamoto N;Rosser CJ
最近的流行病学，遗传学和分子研究表明，感染和炎症引发某些癌症，包括前列腺癌。美国癌症协会估计，全球约20%的癌症是由感染引起的。支原体是一种缺乏细胞壁的细菌，是少数几种可以与哺乳动物细胞密切生长的原核生物之一，通常没有任何明显的病理学，长时间。在这项研究中，生殖支原体，一种流行的性传播感染，和支原体hyorhinis，在艾滋病患者中发现异常高的频率，诱导良性人类前列腺细胞（BPH-1）的恶性表型的能力进行了评估，使用一系列的体外和体内试验。培养19周后，感染的BPH-1细胞实现了锚定非依赖性生长，并增加了迁移和侵袭。通过在无胸腺小鼠中形成异种移植瘤证实了感染的BPH-1细胞的恶性转化。与这些变化相关联的是核型熵的增加，明显的是染色体畸变和多体性的积累。这是第一次报道M. genitalium或M.猪鼻病毒感染导致人良性上皮细胞恶性转化，可作为进一步研究前列腺炎与前列腺癌发生关系的模型。
Recent epidemiologic, genetic, and molecular studies suggest infection and inflammation initiate certain cancers, including those of the prostate. The American Cancer Society, estimates that approximately 20% of all worldwide cancers are caused by infection. Mycoplasma, a genus of bacteria that lack a cell wall, are among the few prokaryotes that can grow in close relationship with mammalian cells, often without any apparent pathology, for extended periods of time. In this study, the capacity of Mycoplasma genitalium, a prevalent sexually transmitted infection, and Mycoplasma hyorhinis, a mycoplasma found at unusually high frequency among patients with AIDS, to induce a malignant phenotype in benign human prostate cells (BPH-1) was evaluated using a series of in vitro and in vivo assays. After 19 weeks of culture, infected BPH-1 cells achieved anchorage-independent growth and increased migration and invasion. Malignant transformation of infected BPH-1 cells was confirmed by the formation of xenograft tumors in athymic mice. Associated with these changes was an increase in karyotypic entropy, evident by the accumulation of chromosomal aberrations and polysomy. This is the first report describing the capacity of M. genitalium or M. hyorhinis infection to lead to the malignant transformation of benign human epithelial cells and may serve as a model to further study the relationship between prostatitis and prostatic carcinogenesis.