The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin
The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin
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DOI：
10.1242/jcs.064774
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发表时间：
2010-09-15
影响因子：
4
通讯作者：
Hearing, Vincent J.
中科院分区：
文献类型：
--
作者：
Choi, Wonseon;Wolber, Rainer;Hearing, Vincent J.
皮肤中黑素细胞和邻近细胞之间的相互作用在调节人类皮肤颜色方面很重要。我们最近证明，手掌和脚底的色素较少和较厚的皮肤是由这些区域的潜在成纤维细胞调节的，特别是通过调节Wnt信号的分泌因子（DKK 1）。在这项研究中，我们测试的假设，真皮成纤维细胞调节组成的肤色的个人，从非常轻，非常深。我们使用微阵列分析来比较来自较浅皮肤类型与较深皮肤类型的成纤维细胞的基因表达模式，重点是分泌蛋白。我们确定了许多基因的表达显着不同，并在表达的蛋白质，神经调节蛋白-1，这是由来自深色皮肤的成纤维细胞分泌，有效地增加了色素沉着的黑色素细胞在组织培养和人工皮肤模型，并调节其生长，这表明它是决定人类皮肤颜色的主要因素之一。
Interactions between melanocytes and neighboring cells in the skin are important in regulating skin color in humans. We recently demonstrated that the less pigmented and thicker skin on the palms and soles is regulated by underlying fibroblasts in those areas, specifically via a secreted factor (DKK1) that modulates Wnt signaling. In this study, we tested the hypothesis that dermal fibroblasts regulate the constitutive skin color of individuals ranging from very light to very dark. We used microarray analysis to compare gene expression patterns in fibroblasts derived from lighter skin types compared to darker skin types, with a focus on secreted proteins. We identified a number of genes that differ dramatically in expression and, among the expressed proteins, neuregulin-1, which is secreted by fibroblasts derived from dark skin, effectively increases the pigmentation of melanocytes in tissue culture and in an artificial skin model and regulates their growth, suggesting that it is one of the major factors determining human skin color.