Enhanced production of nukacin D13E in Lactococcus lactis NZ9000 by the additional expression of immunity genes
Enhanced production of nukacin D13E in Lactococcus lactis NZ9000 by the additional expression of immunity genes
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通过免疫基因的额外表达增强乳酸乳球菌 NZ9000 中 nukacin D13E 的产量
DOI：
10.1007/s00253-011-3563-1
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
et al
中科院分区：
文献类型：
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作者：
Tijo Varghese Puramattathu;et al
努卡星D13 E（D13 E）是由瓦氏葡萄球菌ISK-1产生的A（II）型羊毛硫醚抗生素努卡星ISK-1的变体。D13 E对多种革兰氏阳性菌的特异性抗微生物活性比Nukacin ISK-1高两倍。我们曾报道过乳酸链球菌NZ 9000在乳酸链球菌素控制的基因表达系统的控制下异源生产D13 E。在这项研究中，我们证明了通过额外表达免疫基因nukFEG来提高D13 E的产量。由ABC转运蛋白复合物NukFEG和乳酸结合蛋白NukH赋予的Nukacin ISK-1免疫力不会被D13 E压倒。额外的NukFEG导致菌株的免疫水平增加了4倍，D13 E产量增加了5.2倍。具有最高D13 E免疫力的另外的NukFEGH表达菌株显示出降低的生产水平。通过使用pH控制的分批发酵实现了D13 E生产的进一步改进。
Nukacin D13E (D13E) is a variant of type-A(II) lantibiotic nukacin ISK-1 produced byStaphylococcus warneriISK-1. D13E exhibited a twofold higher specific antimicrobial activity than nukacin ISK-1 against a number of Gram-positive bacteria. We previously reported the heterologous production of D13E inLactococcus lactisNZ9000 under the control of nisin-controlled gene expression system. In this study, we demonstrated enhanced production of D13E by the additional expression of immunity genes,nukFEG. The nukacin ISK-1 immunity, conferred by the ABC transporter complex, NukFEG, and the lantibiotic-binding protein, NukH, was not overwhelmed by D13E. The additional NukFEG resulted in a fourfold increase in the immunity level of the strain and a 5.2-fold increase in D13E production. The additional NukFEGH-expressing strain with the highest D13E immunity showed reduced level of production. Further improvement in D13E production was achieved by using pH-controlled batch fermentation.