Genetic organization of Bungarus multicinctus protease inhibitor-like proteins
Genetic organization of Bungarus multicinctus protease inhibitor-like proteins
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DOI：
10.1016/j.toxicon.2008.03.025
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发表时间：
2008-06-15
期刊：
影响因子：
2.8
通讯作者：
Chou, Wen-Min
中科院分区：
文献类型：
--
作者：
Chang, Long-sen;Wang, Jeh-Jeng;Chou, Wen-Min
本研究报告了编码金环蛇蛋白酶抑制剂样蛋白 (PILP)、PILP-1、PILP-2 和 PILP-3 的基因的结构组织。与 PILP-2 和 PILP-3 不同，重组 PILP-1 对胰蛋白酶表现出抑制活性。 PILP基因和B链基因具有相同的组织结构，三个外显子被相似位置的两个内含子打断。相反，这些基因的内含子1具有相似的大小，观察到内含子2的大小存在显着变化。结果发现，PILP基因中B1链和B2链基因第二内含子的两个区域缺失。值得注意的是，B链基因第二个内含子的内含子插入出现在PILP-1基因的启动子区，但没有出现在PILP-2和PILP-3基因的启动子区。 PILP基因和B链基因的比较分析表明，除了信号肽结构域外，外显子的蛋白质编码区比内含子更加多样化。这些结果表明，PILP 基因和 B 链基因起源于共同的祖先，并且加速进化可能使 PILP 和 B 链基因多样化，正如蛇毒磷脂酶 A(2)、神经毒素和心脏毒素基因所提出的那样。 (c) 2008 Elsevier Ltd. 保留所有权利。
The structural organization of the genes encoding Bungarus multicinctus protease inhibitor-like proteins (PILPs), PILP-1, PILP-2 and PILP-3, are reported in this study. Unlike PILP-2 and PILP-3, recombinant PILP-1 exhibited inhibitory activity on trypsin. PILP genes and B chain genes shared identical organization with three exons interrupted by two introns in similar positions. On the contrary, intron 1 of these genes had a similar size, a notable variation with the size of intron 2 was observed. It was found that two regions at the second intron of B1 chain and B2 chain genes were absent in that of PILP genes. Noticeably, intronic insertion in the second intron of B chain genes appeared in the promoter region of PILP-1 gene, but not in that of PILP-2 and PILP-3 genes. Comparative analyses of PILP genes and B chain genes showed that the protein-coding regions of the exons are more diverse than introns, except for in the signal peptide domain. These results suggest that PILP genes and B chain genes originate from a common ancestor, and that accelerated evolution may diversify PILP and B chain genes as that proposed for snake venom phospholipase A(2), neurotoxin and cardiotoxin genes. (c) 2008 Elsevier Ltd. All rights reserved.