Rat embryonic stem-like (ES-like) cells can contribute to extraembryonic tissues in vivo
Rat embryonic stem-like (ES-like) cells can contribute to extraembryonic tissues in vivo
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DOI：
10.1089/clo.2007.0029
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发表时间：
2007-12-01
期刊：
影响因子：
--
通讯作者：
Smith, Lawrence C.
中科院分区：
文献类型：
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作者：
Demers, Simon-Pierre;Yoo, Jae Gyu;Smith, Lawrence C.
尽管通过小鼠胚胎干 (ES) 细胞的基因靶向取得了重大进展，但该技术目前仅适用于小鼠。由于大鼠对于生物医学研究具有不可否认的重要性，因此多年来一直在尝试衍生大鼠 ES 细胞系。然而，迄今为止报道的假定的大鼠ES细胞系尚未表现出在胚胎注射后在体内促进组织发育的能力。与之前的研究相比，我们在此描述了大鼠ES样细胞系的成功衍生和表征，该细胞系不仅表达未分化细胞的标记、碱性磷酸酶（AP）活性和阶段特异性胚胎抗原1（SSEA-1）细胞表面抗原，而且还保留Oct4（也称为Pou5f1）的表达，Oct4是同源域转录因子和多能细胞的分子标记。值得注意的是，这些大鼠 ES 样细胞在注射到转移到假孕雌性体内的囊胚中时，可以促进胚胎外组织的发育。该报告首次证明，大鼠ES样细胞可以有效衍生，可以表达一组多能细胞标志物，可以在体外进行基因修饰和冷冻保存，而且重要的是，能够在体内促进胚胎外组织。
Despite significant Advances achieved through gene targeting in mouse embryonic stem (ES) cells, this technology is presently only available in mice. Because the rat is a species of undeniable importance to biomedical research, attempts at derivation of rat ES cell lines have been ongoing for many years; however, the putative rat ES cell lines that have been reported to date have not yet displayed the ability to contribute in vivo to developing tissues following embryo injection. In contrast to previous studies, we describe herein the successful derivation and characterization of rat ES-like cell lines that not only express markers of undifferentiated cells, alkaline phosphatase (AP) activity and stage-specific embryonic antigen-1 (SSEA-1) cell surface antigen, but also retain expression of Oct4 (also known as Pou5f1) a homeodomain transcription factor and molecular marker of pluripotent cells. Notably, these rat ES-like cells, when injected into blastocysts transferred to pseudopregnant females, can contribute to developing extraembryonic tissues. This report demonstrates for the first time that rat ES-like cells can be derived efficiently, can express a panel of pluripotent cell markers, can be genetically modified in vitro and cryopreserved, and importantly, are capable of contributing to extraembryonic tissues in vivo.