RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE AND FRUIT-SET OR DEGENERATION OF UNPOLLINATED OVARIES OF PISUM-SATIVUM-L
RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE AND FRUIT-SET OR DEGENERATION OF UNPOLLINATED OVARIES OF PISUM-SATIVUM-L
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DOI：
10.1007/bf00395972
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发表时间：
1985-01-01
期刊：
影响因子：
4.3
通讯作者：
GARCIAMARTINEZ, JL
中科院分区：
文献类型：
--
作者：
CARBONELL, J;GARCIAMARTINEZ, JL
豌豆（P. sativumL.）研究了发育或退化早期的卵巢。未授粉子房的发育受到不同植物生长调节剂（GA，2，4-D和N6-苄基腺嘌呤）或植物打顶的刺激。在刺激卵巢的所有类型的自消化提取物中，均可检测到与核酮糖-1，5-二磷酸羧化酶（RuBPCase）亚基（16和55 kDa [千道尔顿]）具有相似迁移率的多肽条带。然而，这些带是不存在的autodestigated提取物从未受刺激的卵巢开花后3天后，这些提取物的autodestigated混合物的模式，无论是从受刺激的卵巢或那些fron未受刺激的卵巢前3天的电泳模式。开花后3天左右，未受刺激的子房出现促进RuBPCase水解的水解活性。这一事件与未授粉的子房响应GA的发育能力的丧失和子房壁的退化相一致。
The polypeptide patterns obtained by sodium dodeceylsulfate-polyacrylamide gel electrophoresis of undigested and autodigested extracts from pea (P. sativum L.) ovaries at the early stages of development or degeneration were studied. Development of unpollinated ovaries was stimulated by application of different plant growth regulators (GA, 2,4-D and N6-benzyladenine) or by plant topping. Polypeptide bands of similar mobility to ribulose-1,5-bisphosphate carboxylase (RuBPCase) subunits (16 and 55 kDa [kilodalton]) could be detected in all types of autodigested extracts from stimulated ovaries. However, these bands were absent in electrophoretic patterns of autodigested extracts from unstimulated ovaries after 3 days post anthesis and in patterns of autodigested mixtures of these extracts with either those from stimulated ovaries or those fron unstimulated ovaries before day 3. A poteolytic activity which promotes the hydrolysis of RuBPCase appears in unstimulated ovaries about 3 days after anthesis. This event coincides with the loss of the capacity of unpollinated ovaries to develop in response to GA and with the degeneration of the ovary wall.