Circulating tumor cells using hTERT-specific replication-selective adenovirus in patients with soft tissue sarcoma
Circulating tumor cells using hTERT-specific replication-selective adenovirus in patients with soft tissue sarcoma
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发表时间：
2017
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通讯作者：
Toshihiro Matsuo;M. Deie;T. Sugita;S. Shimose;Y. Urata;Norimitsu Wakao;Katsuhisa Kawanami;M. Ochi
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作者：
Toshihiro Matsuo;M. Deie;T. Sugita;S. Shimose;Y. Urata;Norimitsu Wakao;Katsuhisa Kawanami;M. Ochi
外周血中循环肿瘤细胞（CTC）的存在为测量各种癌症的治疗效果提供了有用的预后因素和工具。我们尝试使用端粒酶特异性病毒试剂检测肉瘤患者外周血中的活CTC。我们研究了CTC数量与肉瘤其他临床特征之间的相关性。对于CTC分析，从10名躯干或四肢软组织肉瘤患者手术前后获得20份血液样本。根据法国癌症中心联合会肉瘤组系统，5例患者被诊断为2级肉瘤，5例被诊断为3级肉瘤。平均术后随访36.6个月（范围：12-49个月）。肿瘤预后如下：6例患者保持无病，2例患者无疾病证据，2例患者死于疾病。3例患者术后发生肺转移。我们将7.5 ml血液样本与携带绿色荧光蛋白（GFP）基因的端粒酶特异性、复制选择性、溶瘤腺病毒试剂一起孵育，这允许检测活的CTC。使用荧光显微镜对GFP阳性细胞进行计数。术前（1.9;范围，0-6）和术后（2.6;范围，0-18; P=0.704）的CTC平均数量无显著差异。与术前相比，所有术后CTCs数量增加的患者均表现出肺转移。术后CTC数量与肿瘤转移（P=0.022）和生存预后（P=0.014）显著相关。恶性肿瘤中CTC的数量与肺转移的发生及预后有显著相关性。使用端粒酶特异性病毒试剂检测CTC可能被证明对预后评估有用。
The presence of circulating tumor cells (CTCs) in peripheral blood offers a useful prognostic factor and tool for measuring the effects of treatment for various carcinomas. We attempted to detect viable CTCs in peripheral blood from sarcoma patients using a telomerase-specific viral agent. We examined correlations between numbers of CTCs and other clinical features of sarcoma. For CTC analysis, 20 blood samples were obtained from 10 patients with soft-tissue sarcoma of the trunk or extremities before and after surgery. Five patients were diagnosed with grade 2 sarcoma and five were diagnosed with grade 3 sarcoma according to the French Federation of Cancer Centers Sarcoma Group System. Mean postoperative follow-up was 36.6 months (range, 12-49 months). Oncological prognosis was as follows: 6 patients remained continuously disease-free, 2 patients showed no evidence of disease, and 2 patients died of the disease. Three patients developed lung metastases after surgery. We incubated 7.5-ml blood samples with a telomerase-specific, replication-selective, oncolytic adenoviral agent carrying the green fluorescent protein (GFP) gene, which allowed for the detection of viable CTCs. GFP-positive cells were counted using fluorescence microscopy. The mean number of CTCs showed no significant difference between preoperatively (1.9; range, 0-6) and postoperatively (2.6; range, 0-18; P=0.704). All patients with increased numbers of CTCs postoperatively compared to preoperatively displayed lung metastases. The number of postoperative CTCs correlated significantly with metastasis (P=0.022) and life prognosis (P=0.014). A significant relationship was found between the number of CTCs and both occurrence of lung metastasis and prognosis in patients with sarcoma. Detection of CTCs using telomerase-specific viral agent may prove useful for prognostic evaluation.